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1nzb

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[[Image:1nzb.gif|left|200px]]
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{{STRUCTURE_1nzb| PDB=1nzb | SCENE= }}
{{STRUCTURE_1nzb| PDB=1nzb | SCENE= }}
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'''Crystal structure of wild type Cre recombinase-loxP synapse'''
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===Crystal structure of wild type Cre recombinase-loxP synapse===
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==Overview==
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Escherichia coli phage P1 Cre recombinase catalyzes the site-specific recombination of DNA containing loxP sites. We report here two crystal structures of a wild-type Cre recombinase-loxP synaptic complex corresponding to two distinct reaction states: an initial pre-cleavage complex, trapped using a phosphorothioate modification at the cleavable scissile bond that prevents the recombination reaction, and a 3'-phosphotyrosine protein-DNA intermediate resulting from the first strand cleavage. In contrast to previously determined Cre complexes, both structures contain a full tetrameric complex in the asymmetric unit, unequivocally showing that the anti-parallel arrangement of the loxP sites is an intrinsic property of the Cre-loxP recombination synapse. The conformation of the spacer is different to the one observed for the symmetrized loxS site: a kink next to the scissile phosphate in the top strand of the pre-cleavage complex leads to unstacking of the TpG step and a widening of the minor groove. This side of the spacer is interacting with a 'cleavage-competent' Cre subunit, suggesting that the first cleavage occurs at the ApT step in the top strand. This is further confirmed by the structure of the 3'-phosphotyrosine intermediate, where the DNA is cleaved in the top strands and covalently linked to the 'cleavage-competent' subunits. The cleavage is followed by a movement of the C-terminal part containing the attacking Y324 and the helix N interacting with the 'non-cleaving' subunit. This rearrangement could be responsible for the interconversion of Cre subunits. Our results also suggest that the Cre-induced kink next to the scissile phosphodiester activates the DNA for cleavage at this position and facilitates strand transfer.
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(as it appears on PubMed at http://www.pubmed.gov), where 12954782 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12954782}}
==About this Structure==
==About this Structure==
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[[Category: Dna]]
[[Category: Dna]]
[[Category: Recombinase]]
[[Category: Recombinase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:10:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:07:52 2008''

Revision as of 11:08, 27 July 2008

Image:1nzb.png

Template:STRUCTURE 1nzb

Crystal structure of wild type Cre recombinase-loxP synapse

Template:ABSTRACT PUBMED 12954782

About this Structure

1NZB is a Single protein structure of sequence from Enterobacteria phage p1. Full crystallographic information is available from OCA.

Reference

Crystal structure of a wild-type Cre recombinase-loxP synapse reveals a novel spacer conformation suggesting an alternative mechanism for DNA cleavage activation., Ennifar E, Meyer JE, Buchholz F, Stewart AF, Suck D, Nucleic Acids Res. 2003 Sep 15;31(18):5449-60. PMID:12954782

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