1nzj

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{{STRUCTURE_1nzj| PDB=1nzj | SCENE= }}
{{STRUCTURE_1nzj| PDB=1nzj | SCENE= }}
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'''Crystal Structure and Activity Studies of Escherichia Coli Yadb ORF'''
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===Crystal Structure and Activity Studies of Escherichia Coli Yadb ORF===
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==Overview==
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In the course of a structural genomics program aiming at solving the structures of Escherichia coli open reading frame products of unknown function, we have determined the structure of YadB at 1.5A using molecular replacement. The YadB protein is 298 amino acid residues long and displays 34% sequence identity with E.coli glutamyl-tRNA synthetase (GluRS). It is much shorter than GluRS, which contains 468 residues, and lacks the complete domain interacting with the tRNA anticodon loop. As E.coli GluRS, YadB possesses a Zn2+ located in the putative tRNA acceptor stem-binding domain. The YadB cluster uses cysteine residues as the first three zinc ligands, but has a weaker tyrosine ligand at the fourth position. It shares with canonical amino acid RNA synthetases a major functional feature, namely activation of the amino acid (here glutamate). It differs, however, from GluRSs by the fact that the activation step is tRNA-independent and that it does not catalyze attachment of the activated glutamate to E.coli tRNAGlu, but to another, as yet unknown tRNA. These results suggest thus a novel function, distinct from that of GluRSs, for the yadB gene family.
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(as it appears on PubMed at http://www.pubmed.gov), where 15003446 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15003446}}
==About this Structure==
==About this Structure==
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[[Category: Structural genomic]]
[[Category: Structural genomic]]
[[Category: Zn cluster]]
[[Category: Zn cluster]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:10:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:40:28 2008''

Revision as of 10:40, 27 July 2008

Image:1nzj.png

Template:STRUCTURE 1nzj

Crystal Structure and Activity Studies of Escherichia Coli Yadb ORF

Template:ABSTRACT PUBMED 15003446

About this Structure

1NZJ is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

The Escherichia coli YadB gene product reveals a novel aminoacyl-tRNA synthetase like activity., Campanacci V, Dubois DY, Becker HD, Kern D, Spinelli S, Valencia C, Pagot F, Salomoni A, Grisel S, Vincentelli R, Bignon C, Lapointe J, Giege R, Cambillau C, J Mol Biol. 2004 Mar 19;337(2):273-83. PMID:15003446

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