8us4

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m (Protected "8us4" [edit=sysop:move=sysop])
Current revision (12:46, 17 July 2024) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 8us4 is ON HOLD until Paper Publication
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==C2221 Crystal structure of TamA (Barrel only) from Pseudomonas aeruginosa at 3.15 Ang==
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<StructureSection load='8us4' size='340' side='right'caption='[[8us4]], [[Resolution|resolution]] 3.15&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[8us4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8US4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8US4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.15&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PT:PLATINUM+(II)+ION'>PT</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8us4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8us4 OCA], [https://pdbe.org/8us4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8us4 RCSB], [https://www.ebi.ac.uk/pdbsum/8us4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8us4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9I0U1_PSEAE Q9I0U1_PSEAE]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The outer membrane (OM) of gram-negative bacteria serves as a vital organelle that is densely populated with OM proteins (OMPs) and plays pivotal roles in cellular functions and virulence. The assembly and insertion of these OMPs into the OM represent a fundamental process requiring specialized molecular chaperones. One example is the translocation and assembly module (TAM), which functions as a transenvelope chaperone promoting the folding of specific autotransporters, adhesins, and secretion systems. The catalytic unit of TAM, TamA, comprises a catalytic beta-barrel domain anchored within the OM and three periplasmic polypeptide-transport-associated (POTRA) domains that recruit the TamB subunit. The latter acts as a periplasmic ladder that facilitates the transport of unfolded OMPs across the periplasm. In addition to their role in recruiting the auxiliary protein TamB, our data demonstrate that the POTRA domains mediate interactions with the inner surface of the OM, ultimately modulating the membrane properties. Through the integration of X-ray crystallography, molecular dynamic simulations, and biomolecular interaction methodologies, we located the membrane-binding site on the first and second POTRA domains. Our data highlight a binding preference for phosphatidylglycerol, a minor lipid constituent present in the OM, which has been previously reported to facilitate OMP assembly. In the context of the densely OMP-populated membrane, this association may serve as a mechanism to secure lipid accessibility for nascent OMPs through steric interactions with existing OMPs, in addition to creating favorable conditions for OMP biogenesis.
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Authors:
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POTRA domains of the TamA insertase interact with the outer membrane and modulate membrane properties.,Mellouk A, Jaouen P, Ruel LJ, Le M, Martini C, Moraes TF, El Bakkouri M, Lague P, Boisselier E, Calmettes C Proc Natl Acad Sci U S A. 2024 Jul 9;121(28):e2402543121. doi: , 10.1073/pnas.2402543121. Epub 2024 Jul 3. PMID:38959031<ref>PMID:38959031</ref>
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Description:
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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<div class="pdbe-citations 8us4" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Pseudomonas aeruginosa PAO1]]
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[[Category: Calmettes C]]
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[[Category: Mellouk A]]
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[[Category: Moraes TF]]

Current revision

C2221 Crystal structure of TamA (Barrel only) from Pseudomonas aeruginosa at 3.15 Ang

PDB ID 8us4

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