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1o5t

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{{STRUCTURE_1o5t| PDB=1o5t | SCENE= }}
{{STRUCTURE_1o5t| PDB=1o5t | SCENE= }}
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'''Crystal structure of the aminoacylation catalytic fragment of human tryptophanyl-tRNA synthetase'''
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===Crystal structure of the aminoacylation catalytic fragment of human tryptophanyl-tRNA synthetase===
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==Overview==
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Human tryptophanyl-tRNA synthetase (hTrpRS) produces a full-length and three N terminus-truncated forms through alternative splicing and proteolysis. The shortest fragment that contains the aminoacylation catalytic fragment (T2-hTrpRS) exhibits the most potent angiostatic activity. We report here the crystal structure of T2-hTrpRS at 2.5 A resolution, which was solved using the multi-wavelength anomalous diffraction method. T2-hTrpRS shares a very low sequence homology of 22% with Bacillus stearothermophilus TrpRS (bTrpRS); however, their overall structures are strikingly similar. Structural comparison of T2-hTrpRS with bTrpRS reveals substantial structural differences in the substrate-binding pocket and at the entrance to the pocket that play important roles in substrate binding and tRNA binding. T2-hTrpRS has a wide opening to the active site and adopts a compact conformation similar to the closed conformation of bTrpRS. These results suggest that mammalian and bacterial TrpRSs might use different mechanisms to recognize the substrate. Modeling studies indicate that tRNA binds with the dimeric enzyme and interacts primarily with the connective polypeptide 1 of hTrpRS via its acceptor arm and the alpha-helical domain of hTrpRS via its anticodon loop. Our results also suggest that the angiostatic activity is likely located at the alpha-helical domain, which resembles the short chain cytokines.
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==About this Structure==
==About this Structure==
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[[Category: Four helix bundle]]
[[Category: Four helix bundle]]
[[Category: Rossmann fold]]
[[Category: Rossmann fold]]
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Revision as of 13:18, 5 July 2008

Image:1o5t.png

Template:STRUCTURE 1o5t

Crystal structure of the aminoacylation catalytic fragment of human tryptophanyl-tRNA synthetase

Template:ABSTRACT PUBMED 14660560

About this Structure

1O5T is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of human tryptophanyl-tRNA synthetase catalytic fragment: insights into substrate recognition, tRNA binding, and angiogenesis activity., Yu Y, Liu Y, Shen N, Xu X, Xu F, Jia J, Jin Y, Arnold E, Ding J, J Biol Chem. 2004 Feb 27;279(9):8378-88. Epub 2003 Dec 5. PMID:14660560

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