1o7f

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{{Seed}}
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{{STRUCTURE_1o7f| PDB=1o7f | SCENE= }}
{{STRUCTURE_1o7f| PDB=1o7f | SCENE= }}
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'''CRYSTAL STRUCTURE OF THE REGULATORY DOMAIN OF EPAC2'''
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===CRYSTAL STRUCTURE OF THE REGULATORY DOMAIN OF EPAC2===
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==Overview==
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Cyclic adenosine monophosphate (cAMP) is a universal second messenger that, in eukaryotes, was believed to act only on cAMP-dependent protein kinase A (PKA) and cyclic nucleotide-regulated ion channels. Recently, guanine nucleotide exchange factors specific for the small GTP-binding proteins Rap1 and Rap2 (Epacs) were described, which are also activated directly by cAMP. Here, we have determined the three-dimensional structure of the regulatory domain of Epac2, which consists of two cyclic nucleotide monophosphate (cNMP)-binding domains and one DEP (Dishevelled, Egl, Pleckstrin) domain. This is the first structure of a cNMP-binding domain in the absence of ligand, and comparison with previous structures, sequence alignment and biochemical experiments allow us to delineate a mechanism for cyclic nucleotide-mediated conformational change and activation that is most likely conserved for all cNMP-regulated proteins. We identify a hinge region that couples cAMP binding to a conformational change of the C-terminal regions. Mutations in the hinge of Epac can uncouple cAMP binding from its exchange activity.
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(as it appears on PubMed at http://www.pubmed.gov), where 12469113 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12469113}}
==About this Structure==
==About this Structure==
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[[Category: Gef]]
[[Category: Gef]]
[[Category: Regulation]]
[[Category: Regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:28:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:21:38 2008''

Revision as of 12:21, 29 July 2008

Image:1o7f.png

Template:STRUCTURE 1o7f

CRYSTAL STRUCTURE OF THE REGULATORY DOMAIN OF EPAC2

Template:ABSTRACT PUBMED 12469113

About this Structure

1O7F is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

Structure and regulation of the cAMP-binding domains of Epac2., Rehmann H, Prakash B, Wolf E, Rueppel A, de Rooij J, Bos JL, Wittinghofer A, Nat Struct Biol. 2003 Jan;10(1):26-32. PMID:12469113

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