1o7l

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{{STRUCTURE_1o7l| PDB=1o7l | SCENE= }}
{{STRUCTURE_1o7l| PDB=1o7l | SCENE= }}
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'''MOLYBDATE-ACTIVATED FORM OF MODE FROM ESCHERICHIA COLI'''
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===MOLYBDATE-ACTIVATED FORM OF MODE FROM ESCHERICHIA COLI===
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==Overview==
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ModE is a bacterial transcriptional regulator that orchestrates many aspects of molybdenum metabolism by binding to specific DNA sequences in a molybdate-dependent fashion. We present the crystal structure of Escherichia coli ModE in complex with molybdate, which was determined at 2.75A from a merohedrally twinned crystal (twin fraction approximately 0.30) with space group P4(3). We now have structures of ModE in both its "switched on" (ligand-bound) and "switched off" (apo) states. Comparison with the apo structure shows that ligand binding leads to extensive conformational changes not only in the molybdate-binding domain, but also in the DNA-binding domain. The most obvious difference is the loss of the pronounced asymmetry between the two chains of the ModE dimer, which had been a characteristic property of the apo structure. Another major change concerns the relative orientation of the two DNA-interacting winged helix-turn-helix motifs. Manual docking of an idealized DNA structure suggests that this conformational change should improve DNA binding of the activated molybdate-bound ModE.
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(as it appears on PubMed at http://www.pubmed.gov), where 12581638 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12581638}}
==About this Structure==
==About this Structure==
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[[Category: Transcriptional regulator,activator]]
[[Category: Transcriptional regulator,activator]]
[[Category: Winged helix-turn-helix]]
[[Category: Winged helix-turn-helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:29:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:24:19 2008''

Revision as of 03:24, 29 July 2008

Image:1o7l.png

Template:STRUCTURE 1o7l

MOLYBDATE-ACTIVATED FORM OF MODE FROM ESCHERICHIA COLI

Template:ABSTRACT PUBMED 12581638

About this Structure

1O7L is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of activated ModE reveals conformational changes involving both oxyanion and DNA-binding domains., Schuttelkopf AW, Boxer DH, Hunter WN, J Mol Biol. 2003 Feb 21;326(3):761-7. PMID:12581638

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