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| | + | '''Unreleased structure''' |
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| - | ==Crystal structure of cytochrome P450 CftA from Streptomyces torulosus NRRL B-3889, in complex with the substrate ikarugamycin==
| + | The entry 8jnp is ON HOLD until Paper Publication |
| - | <StructureSection load='8jnp' size='340' side='right'caption='[[8jnp]], [[Resolution|resolution]] 2.00Å' scene=''>
| + | |
| - | == Structural highlights ==
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| - | <table><tr><td colspan='2'>[[8jnp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_torulosus Streptomyces torulosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8JNP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8JNP FirstGlance]. <br>
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| - | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr>
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| - | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EIA:(1Z,3E,5S,7R,8R,10R,11R,12S,15R,16S,18Z,25S)-11-ethyl-2-hydroxy-10-methyl-21,26-diazapentacyclo[23.2.1.05,16.07,15.08,12]octacosa-1(2),3,13,18-tetraene-20,27,28-trione'>EIA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8jnp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8jnp OCA], [https://pdbe.org/8jnp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8jnp RCSB], [https://www.ebi.ac.uk/pdbsum/8jnp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8jnp ProSAT]</span></td></tr>
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| - | </table>
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| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Regio- and chemoselective C-H activation at multi-positions of a single molecule is fascinating but chemically challenging. The homologous cytochrome P450 enzymes IkaD and CftA catalyze multiple C-H oxidations on the same polycyclic tetramate macrolactam (PoTeM) ikarugamycin, with distinct regio- and chemoselectivity. Herein we provide mechanistic understanding of their functional differences by solving crystal structures of IkaD and CftA in complex with ikarugamycin and unnatural substrates. Distinct conformations of the F/G region in IkaD and CftA are found to differentiate the orientation of PoTeM substrates, by causing different binding patterns with polar moieties to determine site selection, oxidation order, and chemoselectivity. Fine-tuning the polar subpocket altered the regioselectivity of IkaD, indicating that substrate re-orientation by mutating residues distal to the oxidation site could serve as an important method in future engineering of P450 enzymes.
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| - | A Mechanistic Understanding of the Distinct Regio- and Chemoselectivity of Multifunctional P450s by Structural Comparison of IkaD and CftA Complexed with Common Substrates.,Jiang P, Jin H, Zhang G, Zhang W, Liu W, Zhu Y, Zhang C, Zhang L Angew Chem Int Ed Engl. 2023 Nov 2:e202310728. doi: 10.1002/anie.202310728. PMID:37917570<ref>PMID:37917570</ref>
| + | Authors: Jiang, P., Zhang, L.P., Zhang, C.S. |
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | Description: Crystal structure of cytochrome P450 CftA from Streptomyces torulosus NRRL B-3889, in complex with the substrate ikarugamycin |
| - | </div>
| + | [[Category: Unreleased Structures]] |
| - | <div class="pdbe-citations 8jnp" style="background-color:#fffaf0;"></div>
| + | [[Category: Zhang, C.S]] |
| - | == References ==
| + | [[Category: Jiang, P]] |
| - | <references/>
| + | [[Category: Zhang, L.P]] |
| - | __TOC__
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| - | </StructureSection>
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| - | [[Category: Large Structures]] | + | |
| - | [[Category: Streptomyces torulosus]] | + | |
| - | [[Category: Jiang P]] | + | |
| - | [[Category: Zhang CS]] | + | |
| - | [[Category: Zhang LP]]
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Description: Crystal structure of cytochrome P450 CftA from Streptomyces torulosus NRRL B-3889, in complex with the substrate ikarugamycin
