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| | + | '''Unreleased structure''' |
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| - | ==Porcine epidemic diarrhea virus complete core polymerase complex==
| + | The entry 8urb is ON HOLD |
| - | <StructureSection load='8urb' size='340' side='right'caption='[[8urb]], [[Resolution|resolution]] 3.40Å' scene=''>
| + | |
| - | == Structural highlights ==
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| - | <table><tr><td colspan='2'>[[8urb]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Porcine_epidemic_diarrhea_virus Porcine epidemic diarrhea virus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8URB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8URB FirstGlance]. <br>
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| - | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.4Å</td></tr>
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| - | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8urb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8urb OCA], [https://pdbe.org/8urb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8urb RCSB], [https://www.ebi.ac.uk/pdbsum/8urb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8urb ProSAT]</span></td></tr>
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| - | </table>
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| - | == Function ==
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| - | [https://www.uniprot.org/uniprot/U6BRU0_9ALPC U6BRU0_9ALPC]
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| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Coronaviruses are a diverse subfamily of viruses containing pathogens of humans and animals. This subfamily of viruses replicates their RNA genomes using a core polymerase complex composed of viral non-structural proteins: nsp7, nsp8 and nsp12. Most of our understanding of coronavirus molecular biology comes from the betacoronaviruses like SARS-CoV and SARS-CoV-2, the latter of which is the causative agent of COVID-19. In contrast, members of the alphacoronavirus genus are relatively understudied despite their importance in human and animal health. Here we have used cryo-electron microscopy to determine the structure of the alphacoronavirus porcine epidemic diarrhea virus (PEDV) core polymerase complex bound to RNA. Our structure shows an unexpected nsp8 stoichiometry in comparison to other published coronavirus polymerase structures. Biochemical analysis shows that the N-terminal extension of one nsp8 is not required for in vitro RNA synthesis for alpha and betacoronaviruses as previously hypothesized. Our work shows the importance of studying diverse coronaviruses to reveal aspects of coronavirus replication while also identifying areas of conservation to be targeted by antiviral drugs.
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| - | An alphacoronavirus polymerase structure reveals conserved co-factor functions.,Anderson TK, Hoferle PJ, Lee KW, Coon JJ, Kirchdoerfer RN bioRxiv. 2023 Mar 16:2023.03.15.532841. doi: 10.1101/2023.03.15.532841. Preprint. PMID:36993498<ref>PMID:36993498</ref>
| + | Authors: Anderson, T.K., Kirchdoerfer, R.N. |
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | Description: Porcine epidemic diarrhea virus complete core polymerase complex |
| - | </div>
| + | [[Category: Unreleased Structures]] |
| - | <div class="pdbe-citations 8urb" style="background-color:#fffaf0;"></div>
| + | [[Category: Kirchdoerfer, R.N]] |
| - | == References ==
| + | [[Category: Anderson, T.K]] |
| - | <references/>
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| - | __TOC__
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| - | </StructureSection>
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| - | [[Category: Large Structures]]
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| - | [[Category: Porcine epidemic diarrhea virus]]
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| - | [[Category: Synthetic construct]] | + | |
| - | [[Category: Anderson TK]] | + | |
| - | [[Category: Kirchdoerfer RN]] | + | |
