1o9d

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{{STRUCTURE_1o9d| PDB=1o9d | SCENE= }}
{{STRUCTURE_1o9d| PDB=1o9d | SCENE= }}
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'''STRUCTURAL VIEW OF A FUNGAL TOXIN ACTING ON A 14-3-3 REGULATORY COMPLEX'''
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===STRUCTURAL VIEW OF A FUNGAL TOXIN ACTING ON A 14-3-3 REGULATORY COMPLEX===
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==Overview==
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The fungal phytotoxin fusicoccin stabilizes the interaction between the C-terminus of the plant plasma membrane H(+)-ATPase and 14-3-3 proteins, thus leading to permanent activation of the proton pump. This results in an irreversible opening of the stomatal pore, followed by wilting of plants. Here, we report the crystal structure of the ternary complex between a plant 14-3-3 protein, fusicoccin and a phosphopeptide derived from the C-terminus of the H(+)-ATPase. Comparison with the corresponding binary 14-3-3 complexes indicates no major conformational change induced by fusicoccin. The compound rather fills a cavity in the protein-phosphopeptide interaction surface. Isothermal titration calorimetry indicates that the toxin alone binds only weakly to 14-3-3 and that peptide and toxin mutually increase each others' binding affinity approximately 90-fold. These results are important for herbicide development but might have general implications for drug development, since rather than inhibiting protein-protein interactions, which is difficult to accomplish, it might be easier to reverse the strategy and stabilize protein-protein complexes. As the fusicoccin interaction shows, only low-affinity interactions would be required for this strategy.
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(as it appears on PubMed at http://www.pubmed.gov), where 12606564 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12606564}}
==About this Structure==
==About this Structure==
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[[Category: Activating drug]]
[[Category: Activating drug]]
[[Category: Fusicoccin]]
[[Category: Fusicoccin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:33:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:55:15 2008''

Revision as of 00:55, 29 July 2008

Image:1o9d.png

Template:STRUCTURE 1o9d

STRUCTURAL VIEW OF A FUNGAL TOXIN ACTING ON A 14-3-3 REGULATORY COMPLEX

Template:ABSTRACT PUBMED 12606564

About this Structure

1O9D is a Protein complex structure of sequences from Nicotiana plumbaginifolia and Nicotiana tabacum. Full crystallographic information is available from OCA.

Reference

Structural view of a fungal toxin acting on a 14-3-3 regulatory complex., Wurtele M, Jelich-Ottmann C, Wittinghofer A, Oecking C, EMBO J. 2003 Mar 3;22(5):987-94. PMID:12606564

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