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1ob9

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{{STRUCTURE_1ob9| PDB=1ob9 | SCENE= }}
{{STRUCTURE_1ob9| PDB=1ob9 | SCENE= }}
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'''HOLLIDAY JUNCTION RESOLVING ENZYME'''
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===HOLLIDAY JUNCTION RESOLVING ENZYME===
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==Overview==
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Two archaeal Holliday junction resolving enzymes, Holliday junction cleavage (Hjc) and Holliday junction endonuclease (Hje), have been characterized. Both are members of a nuclease superfamily that includes the type II restriction enzymes, although their DNA cleaving activity is highly specific for four-way junction structure and not nucleic acid sequence. Despite 28% sequence identity, Hje and Hjc cleave junctions with distinct cutting patterns--they cut different strands of a four-way junction, at different distances from the junction centre. We report the high-resolution crystal structure of Hje from Sulfolobus solfataricus. The structure provides a basis to explain the differences in substrate specificity of Hje and Hjc, which result from changes in dimer organization, and suggests a viral origin for the Hje gene. Structural and biochemical data support the modelling of an Hje:DNA junction complex, highlighting a flexible loop that interacts intimately with the junction centre. A highly conserved serine residue on this loop is shown to be essential for the enzyme's activity, suggesting a novel variation of the nuclease active site. The loop may act as a conformational switch, ensuring that the active site is completed only on binding a four-way junction, thus explaining the exquisite specificity of these enzymes.
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(as it appears on PubMed at http://www.pubmed.gov), where 15479781 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15479781}}
==About this Structure==
==About this Structure==
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[[Category: Nuclease]]
[[Category: Nuclease]]
[[Category: Thermophile]]
[[Category: Thermophile]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:37:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:13:14 2008''

Revision as of 12:13, 27 July 2008

Image:1ob9.png

Template:STRUCTURE 1ob9

HOLLIDAY JUNCTION RESOLVING ENZYME

Template:ABSTRACT PUBMED 15479781

About this Structure

1OB9 is a Single protein structure of sequence from Sulfolobus solfataricus. Full crystallographic information is available from OCA.

Reference

Substrate recognition and catalysis by the Holliday junction resolving enzyme Hje., Middleton CL, Parker JL, Richard DJ, White MF, Bond CS, Nucleic Acids Res. 2004 Oct 12;32(18):5442-51. Print 2004. PMID:15479781

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