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1obu

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{{STRUCTURE_1obu| PDB=1obu | SCENE= }}
{{STRUCTURE_1obu| PDB=1obu | SCENE= }}
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'''APOCRUSTACYANIN C1 CRYSTALS GROWN IN SPACE AND EARTH USING VAPOUR DIFFUSION GEOMETRY'''
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===APOCRUSTACYANIN C1 CRYSTALS GROWN IN SPACE AND EARTH USING VAPOUR DIFFUSION GEOMETRY===
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==Overview==
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Models of apocrustacyanin C(1) were refined against X-ray data recorded on Bending Magnet 14 at the ESRF to resolutions of 1.85 and 2 A from a space-grown and an earth-grown crystal, respectively, both using vapour-diffusion crystal-growth geometry. The space crystals were grown in the APCF on the NASA Space Shuttle. The microgravity crystal growth showed a cyclic nature attributed to Marangoni convection, thus reducing the benefits of the microgravity environment, as reported previously [Chayen et al. (1996), Q. Rev. Biophys. 29, 227-278]. A subsequent mosaicity evaluation, also reported previously, showed only a partial improvement in the space-grown crystals over the earth-grown crystals [Snell et al. (1997), Acta Cryst. D53, 231-239], contrary to the case for lysozyme crystals grown in space with liquid-liquid diffusion, i.e. without any major motion during growth [Snell et al. (1995), Acta Cryst. D52, 1099-1102]. In this paper, apocrustacyanin C(1) electron-density maps from the two refined models are now compared. It is concluded that the electron-density maps of the protein and the bound waters are found to be better overall for the structures of apocrustacyanin C(1) studied from the space-grown crystal compared with those from the earth-grown crystal, even though both crystals were grown using vapour-diffusion crystal-growth geometry. The improved residues are on the surface of the protein, with two involved in or nearby crystal lattice-forming interactions, thus linking an improved crystal-growth mechanism to the molecular level. The structural comparison procedures developed should themselves be valuable for evaluating crystal-growth procedures in the future.
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==About this Structure==
==About this Structure==
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[[Category: Pigment]]
[[Category: Pigment]]
[[Category: Transport protein]]
[[Category: Transport protein]]
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Revision as of 21:42, 27 July 2008

Image:1obu.png

Template:STRUCTURE 1obu

APOCRUSTACYANIN C1 CRYSTALS GROWN IN SPACE AND EARTH USING VAPOUR DIFFUSION GEOMETRY

Template:ABSTRACT PUBMED 12832753

About this Structure

1OBU is a Single protein structure of sequence from Homarus gammarus. Full crystallographic information is available from OCA.

Reference

Apocrustacyanin C(1) crystals grown in space and on earth using vapour-diffusion geometry: protein structure refinements and electron-density map comparisons., Habash J, Boggon TJ, Raftery J, Chayen NE, Zagalsky PF, Helliwell JR, Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1117-23. Epub 2003, Jun 27. PMID:12832753

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