1of9

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{{STRUCTURE_1of9| PDB=1of9 | SCENE= }}
{{STRUCTURE_1of9| PDB=1of9 | SCENE= }}
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'''SOLUTION STRUCTURE OF THE PORE FORMING TOXIN OF ENTAMOEBA HISTOLYTICA (AMOEBAPORE A)'''
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===SOLUTION STRUCTURE OF THE PORE FORMING TOXIN OF ENTAMOEBA HISTOLYTICA (AMOEBAPORE A)===
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==Overview==
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Amoebapore A is a 77-residue protein from the protozoan parasite and human pathogen Entamoeba histolytica. Amoebapores lyse both bacteria and eukaryotic cells by pore formation and play a pivotal role in the destruction of host tissues during amoebiasis, one of the most life-threatening parasitic diseases. Amoebapore A belongs to the superfamily of saposin-like proteins that are characterized by a conserved disulfide bond pattern and a fold consisting of five helices. Membrane-permeabilizing effector molecules of mammalian lymphocytes such as porcine NK-lysin and the human granulysin share these structural attributes. Several mechanisms have been proposed to explain how saposin-like proteins form membrane pores. All mechanisms indicate that the surface charge distribution of these proteins is the basis of their membrane binding capacity and pore formation. Here, we have solved the structure of amoebapore A by NMR spectroscopy. We demonstrate that the specific activation step of amoebapore A depends on a pH-dependent dimerization event and is modulated by a surface-exposed histidine residue. Thus, histidine-mediated dimerization is the molecular switch for pore formation and reveals a novel activation mechanism of pore-forming toxins.
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(as it appears on PubMed at http://www.pubmed.gov), where 14970207 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14970207}}
==About this Structure==
==About this Structure==
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1OF9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OF9 OCA].
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1OF9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OF9 OCA].
==Reference==
==Reference==
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[[Category: Saplip]]
[[Category: Saplip]]
[[Category: Toxin]]
[[Category: Toxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:46:19 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:12:01 2008''

Revision as of 14:12, 28 July 2008

Image:1of9.png

Template:STRUCTURE 1of9

SOLUTION STRUCTURE OF THE PORE FORMING TOXIN OF ENTAMOEBA HISTOLYTICA (AMOEBAPORE A)

Template:ABSTRACT PUBMED 14970207

About this Structure

1OF9 is a Single protein structure of sequence from Entamoeba histolytica. Full experimental information is available from OCA.

Reference

Solution structure of the pore-forming protein of Entamoeba histolytica., Hecht O, Van Nuland NA, Schleinkofer K, Dingley AJ, Bruhn H, Leippe M, Grotzinger J, J Biol Chem. 2004 Apr 23;279(17):17834-41. Epub 2004 Feb 17. PMID:14970207

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