6lh0
From Proteopedia
(Difference between revisions)
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<StructureSection load='6lh0' size='340' side='right'caption='[[6lh0]], [[Resolution|resolution]] 2.81Å' scene=''> | <StructureSection load='6lh0' size='340' side='right'caption='[[6lh0]], [[Resolution|resolution]] 2.81Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'> | + | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LH0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LH0 FirstGlance]. <br> |
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.812Å</td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.812Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A6L:2,3-dihydroxypropyl+(9Z)-octadec-9-enoate'>A6L</scene></td></tr> | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A6L:2,3-dihydroxypropyl+(9Z)-octadec-9-enoate'>A6L</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lh0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lh0 OCA], [https://pdbe.org/6lh0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lh0 RCSB], [https://www.ebi.ac.uk/pdbsum/6lh0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lh0 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lh0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lh0 OCA], [https://pdbe.org/6lh0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lh0 RCSB], [https://www.ebi.ac.uk/pdbsum/6lh0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lh0 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| - | == Function == | ||
| - | [https://www.uniprot.org/uniprot/A0A380PV03_YERFR A0A380PV03_YERFR] | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Apical sodium-dependent bile acid transporter (ASBT) retrieves bile acids from the small intestine and plays a pivotal role in enterohepatic circulation. Currently, high-resolution structures are available for two bacterial ASBT homologs (ASBTNM from Neisseria meningitides and ASBTYf from Yersinia frederiksenii), from which an elevator-style alternating-access mechanism has been proposed for substrate transport. A key concept in this model is that the substrate binds to the central cavity of the transporter so that the elevator-like motion can expose the bound substrate alternatingly to either side of the membrane during a transport cycle. However, no structure of an ASBT has been solved with a substrate bound in its central cavity, so how a substrate binds to ASBT remains to be defined. In this study, molecular docking, structure determination and functional analysis were combined to define and validate the details of substrate binding in ASBTYf. The findings provide coherent evidence to provide a clearer picture of how the substrate binds in the central cavity of ASBTYf that fits the alternating-access model. | ||
| - | |||
| - | Substrate binding in the bile acid transporter ASBTYf from Yersinia frederiksenii.,Wang X, Lyu Y, Ji Y, Sun Z, Zhou X Acta Crystallogr D Struct Biol. 2021 Jan 1;77(Pt 1):117-125. doi:, 10.1107/S2059798320015004. Epub 2021 Jan 1. PMID:33404531<ref>PMID:33404531</ref> | ||
| - | |||
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 6lh0" style="background-color:#fffaf0;"></div> | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Yersinia frederiksenii]] | ||
[[Category: Ji Y]] | [[Category: Ji Y]] | ||
[[Category: Lyu Y]] | [[Category: Lyu Y]] | ||
Current revision
Crystal structure of a cysteine-pair mutant (P10C-S291C) of a bacterial bile acid transporter in an inward-facing apo-state
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Categories: Large Structures | Ji Y | Lyu Y | Sun Z | Wang X | Zhou X
