6m5m
From Proteopedia
(Difference between revisions)
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<StructureSection load='6m5m' size='340' side='right'caption='[[6m5m]], [[Resolution|resolution]] 1.70Å' scene=''> | <StructureSection load='6m5m' size='340' side='right'caption='[[6m5m]], [[Resolution|resolution]] 1.70Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'> | + | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M5M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6M5M FirstGlance]. <br> |
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6m5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m5m OCA], [https://pdbe.org/6m5m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6m5m RCSB], [https://www.ebi.ac.uk/pdbsum/6m5m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6m5m ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6m5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m5m OCA], [https://pdbe.org/6m5m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6m5m RCSB], [https://www.ebi.ac.uk/pdbsum/6m5m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6m5m ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| - | == Function == | ||
| - | [https://www.uniprot.org/uniprot/A0A2Z6G7U6_9BIVA A0A2Z6G7U6_9BIVA] | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | The C-type lectins SPL-1 and SPL-2 from the bivalve Saxidomus purpuratus are composed of A and B chains and of two B chains, respectively. They bind specific carbohydrates containing acetamido groups, such as N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc), in a Ca(2+)-independent manner. Unlike ordinary C-type lectins, which require Ca(2+) ions for carbohydrate recognition, these lectins recognize specific carbohydrates mainly through interactions with the acetamido group without Ca(2+) ions, even though Ca(2+) enhances the binding affinity of these lectins, especially SPL-1. In the present study, the crystal structure of the SPL-1-GlcNAc complex in the presence of Ca(2+) revealed that the binding of SPL-1 to GlcNAc is stabilized by hydrogen bonds to the water molecule(s) coordinating Ca(2+), whereas in ordinary C-type lectins Ca(2+) directly forms coordinate bonds to the hydroxy groups of carbohydrates. These differences may also allow SPL-1 and SPL-2 to recognize both GlcNAc and GalNAc, which have different orientations of the 4-hydroxy group. | ||
| - | |||
| - | Novel carbohydrate-recognition mode of the invertebrate C-type lectin SPL-1 from Saxidomus purpuratus revealed by the GlcNAc-complex crystal in the presence of Ca(2).,Unno H, Higuchi S, Goda S, Hatakeyama T Acta Crystallogr F Struct Biol Commun. 2020 Jun 1;76(Pt 6):271-277. doi:, 10.1107/S2053230X20007256. Epub 2020 Jun 5. PMID:32510468<ref>PMID:32510468</ref> | ||
| - | |||
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 6m5m" style="background-color:#fffaf0;"></div> | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Saxidomus purpurata]] | ||
[[Category: Hatakeyama T]] | [[Category: Hatakeyama T]] | ||
[[Category: Unno H]] | [[Category: Unno H]] | ||
Current revision
SPL-1 - GlcNAc complex
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