1ptu
From Proteopedia
(New page: 200px<br /> <applet load="1ptu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ptu, resolution 2.6Å" /> '''CRYSTAL STRUCTURE OF...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1ptu. | + | [[Image:1ptu.jpg|left|200px]]<br /><applet load="1ptu" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1ptu" size=" | + | |
caption="1ptu, resolution 2.6Å" /> | caption="1ptu, resolution 2.6Å" /> | ||
'''CRYSTAL STRUCTURE OF PROTEIN TYROSINE PHOSPHATASE 1B COMPLEXED WITH PHOSPHOTYROSINE-CONTAINING HEXA-PEPTIDE (DADEPYL-NH2)'''<br /> | '''CRYSTAL STRUCTURE OF PROTEIN TYROSINE PHOSPHATASE 1B COMPLEXED WITH PHOSPHOTYROSINE-CONTAINING HEXA-PEPTIDE (DADEPYL-NH2)'''<br /> | ||
| Line 11: | Line 10: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1PTU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with NH2 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] Full crystallographic information is available from [http:// | + | 1PTU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=NH2:'>NH2</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PTU OCA]. |
==Reference== | ==Reference== | ||
| Line 26: | Line 25: | ||
[[Category: phosphorylation]] | [[Category: phosphorylation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:41:31 2008'' |
Revision as of 14:41, 15 February 2008
|
CRYSTAL STRUCTURE OF PROTEIN TYROSINE PHOSPHATASE 1B COMPLEXED WITH PHOSPHOTYROSINE-CONTAINING HEXA-PEPTIDE (DADEPYL-NH2)
Contents |
Overview
The crystal structures of a cysteine-215-->serine mutant of protein, tyrosine phosphatase 1B complexed with high-affinity peptide substrates, corresponding to an autophosphorylation site of the epidermal growth, factor receptor were determined. Peptide binding to the protein, phosphatase was accompanied by a conformational change of a surface loop, that created a phosphotyrosine recognition pocket and induced a, catalytically competent form of the enzyme. The phosphotyrosine side chain, is buried within the period and anchors the peptide substrate to its, binding site. Hydrogen bonds between peptide main-chain atoms and the, protein contribute to binding affinity, and specific interactions of, acidic residues of the peptide with basic residues on the surface of the, enzyme confer sequence specificity.
Disease
Known diseases associated with this structure: Abdominal body fat distribution, modifier of OMIM:[176885], Insulin resistance, susceptibility to OMIM:[176885]
About this Structure
1PTU is a Single protein structure of sequence from Homo sapiens with as ligand. Active as Protein-tyrosine-phosphatase, with EC number 3.1.3.48 Full crystallographic information is available from OCA.
Reference
Structural basis for phosphotyrosine peptide recognition by protein tyrosine phosphatase 1B., Jia Z, Barford D, Flint AJ, Tonks NK, Science. 1995 Jun 23;268(5218):1754-8. PMID:7540771
Page seeded by OCA on Fri Feb 15 16:41:31 2008
