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| | {{STRUCTURE_1oq0| PDB=1oq0 | SCENE= }} | | {{STRUCTURE_1oq0| PDB=1oq0 | SCENE= }} |
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| - | '''P6.1 stem loop from the activation domain of hTR'''
| + | ===P6.1 stem loop from the activation domain of hTR=== |
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| - | ==Overview==
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| - | The ribonucleoprotein enzyme telomerase maintains chromosome ends in most eukaryotes and is critical for a cell's genetic stability and its proliferative viability. All telomerases contain a catalytic protein component homologous to viral reverse transcriptases (TERT) and an RNA (TR) that provides the template sequence as well as a scaffold for ribonucleoprotein assembly. Vertebrate telomerase RNAs have three essential domains: the template, activation and stability domains. Here we report the NMR structure of an essential RNA element derived from the human telomerase RNA activation domain. The sequence forms a stem-loop structure stabilized by a GU wobble pair formed by two of the five unpaired residues capping a short double helical region. The remaining three loop residues are in a well-defined conformation and form phosphate-base stacking interactions reminiscent of other RNA loop structures. Mutations of these unpaired nucleotides abolish enzymatic activity. The structure rationalizes a number of biochemical observations, and allows us to propose how the loop may function in the telomerase catalytic cycle. The pre-formed structure of the loop exposes the bases of these three essential nucleotides and positions them to interact with other RNA sequences within TR, with the reverse transcriptase or with the newly synthesized telomeric DNA strand. The functional role of this stem-loop appears to be conserved in even distantly related organisms such as yeast and ciliates. | + | The line below this paragraph, {{ABSTRACT_PUBMED_12736311}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 12736311 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_12736311}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OQ0 OCA]. | + | Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OQ0 OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Telomerase]] | | [[Category: Telomerase]] |
| | [[Category: Telomere]] | | [[Category: Telomere]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:08:37 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 02:46:28 2008'' |
Revision as of 23:46, 27 July 2008
Template:STRUCTURE 1oq0
P6.1 stem loop from the activation domain of hTR
Template:ABSTRACT PUBMED 12736311
About this Structure
Full experimental information is available from OCA.
Reference
The solution structure of an essential stem-loop of human telomerase RNA., Leeper T, Leulliot N, Varani G, Nucleic Acids Res. 2003 May 15;31(10):2614-21. PMID:12736311
Page seeded by OCA on Mon Jul 28 02:46:28 2008
