1oqc
From Proteopedia
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{{STRUCTURE_1oqc| PDB=1oqc | SCENE= }} | {{STRUCTURE_1oqc| PDB=1oqc | SCENE= }} | ||
| - | + | ===The crystal structure of augmenter of liver regeneration: a mammalian FAD dependent sulfhydryl oxidase=== | |
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| - | ==Overview== | ||
| - | The crystal structure of recombinant rat augmenter of liver regeneration (ALRp) has been determined to 1.8 A. The protein is a homodimer, stabilized by extensive noncovalent interactions and a network of hydrogen bonds, and possesses a noncovalently bound FAD in a motif previously found only in the related protein ERV2p. ALRp functions in vitro as a disulfide oxidase using dithiothreitol as reductant. Reduction of the flavin by DTT occurs under aerobic conditions resulting in a spectrum characteristic of a neutral semiquinone. This semiquinone is stable and is only fully reduced by addition of dithionite. Mutation of either of two cysteine residues that are located adjacent to the FAD results in inactivation of the oxidase activity. A comparison of ALRp with ERV2p is made that reveals a number of significant structural differences, which are related to the in vivo functions of these two proteins. Possible physiological roles of ALR are examined and a hypothesis that it may serve multiple roles is proposed. | ||
==About this Structure== | ==About this Structure== | ||
| - | 1OQC is a | + | 1OQC is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OQC OCA]. |
==Reference== | ==Reference== | ||
| - | + | <ref group="xtra">PMID:12717032</ref><references group="xtra"/> | |
[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
| - | [[Category: Single protein]] | ||
[[Category: Rose, J P.]] | [[Category: Rose, J P.]] | ||
[[Category: Wang, B C.]] | [[Category: Wang, B C.]] | ||
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[[Category: Liver regeneration]] | [[Category: Liver regeneration]] | ||
[[Category: Sulfhydryl oxidase]] | [[Category: Sulfhydryl oxidase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
| + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 19:39:14 2009'' | ||
Revision as of 17:39, 17 February 2009
The crystal structure of augmenter of liver regeneration: a mammalian FAD dependent sulfhydryl oxidase
About this Structure
1OQC is a 4 chains structure of sequences from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
- Wu CK, Dailey TA, Dailey HA, Wang BC, Rose JP. The crystal structure of augmenter of liver regeneration: A mammalian FAD-dependent sulfhydryl oxidase. Protein Sci. 2003 May;12(5):1109-18. PMID:12717032
Page seeded by OCA on Tue Feb 17 19:39:14 2009
