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| - | [[Image:1or3.jpg|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1or3| PDB=1or3 | SCENE= }} | | {{STRUCTURE_1or3| PDB=1or3 | SCENE= }} |
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| - | '''APOLIPOPROTEIN E3 (APOE3), TRIGONAL TRUNCATION MUTANT 165'''
| + | ===APOLIPOPROTEIN E3 (APOE3), TRIGONAL TRUNCATION MUTANT 165=== |
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| - | ==Overview==
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| - | An amino-terminal fragment of human apolipoprotein E3 (residues 1-165) has been expressed and crystallized in three different crystal forms under similar crystallization conditions. One crystal form has nearly identical cell dimensions to the previously reported orthorhombic (P2(1)2(1)2(1)) crystal form of the amino-terminal 22 kDa fragment of apolipoprotein E (residues 1-191). A second orthorhombic crystal form (P2(1)2(1)2(1) with cell dimensions differing from the first form) and a trigonal (P3(1)21) crystal form were also characterized. The structures of the first orthorhombic and the trigonal form were determined by seleno-methionine multiwavelength anomalous dispersion, and the structure of the second orthorhombic form was determined by molecular replacement using the structure from the trigonal form as a search model. A combination of modern experimental and computational techniques provided high-quality electron-density maps, which revealed new features of the apolipoprotein E structure, including an unambiguously traced loop connecting helices 2 and 3 in the four-helix bundle and a number of multiconformation side chains. The three crystal forms contain a common intermolecular, antiparallel packing arrangement. The electrostatic complimentarity observed in this antiparallel packing resembles the interaction of apolipoprotein E with the monoclonal antibody 2E8 and the low density lipoprotein receptor. Superposition of the model structures from all three crystal forms reveals flexibility and pronounced kinks in helices near one end of the four-helix bundle. This mobility at one end of the molecule provides new insights into the structural changes in apolipoprotein E that occur with lipid association.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_10850798}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 10850798 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_10850798}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Plasma protein]] | | [[Category: Plasma protein]] |
| | [[Category: Vldl]] | | [[Category: Vldl]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:10:50 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 16:17:11 2008'' |
Revision as of 13:17, 29 July 2008
Template:STRUCTURE 1or3
APOLIPOPROTEIN E3 (APOE3), TRIGONAL TRUNCATION MUTANT 165
Template:ABSTRACT PUBMED 10850798
About this Structure
1OR3 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Conformational flexibility in the apolipoprotein E amino-terminal domain structure determined from three new crystal forms: implications for lipid binding., Segelke BW, Forstner M, Knapp M, Trakhanov SD, Parkin S, Newhouse YM, Bellamy HD, Weisgraber KH, Rupp B, Protein Sci. 2000 May;9(5):886-97. PMID:10850798
Page seeded by OCA on Tue Jul 29 16:17:11 2008
