1q1v
From Proteopedia
(New page: 200px<br /> <applet load="1q1v" size="450" color="white" frame="true" align="right" spinBox="true" caption="1q1v" /> '''Structure of the Oncoprotein DEK: a putativ...) |
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'''Structure of the Oncoprotein DEK: a putative DNA-binding Domain Related to the Winged Helix Motif'''<br /> | '''Structure of the Oncoprotein DEK: a putative DNA-binding Domain Related to the Winged Helix Motif'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The chromatin-associated protein DEK was first identified as a fusion | + | The chromatin-associated protein DEK was first identified as a fusion protein in patients with a subtype of acute myelogenous leukemia. It has since become associated with diverse human ailments ranging from cancers to autoimmune diseases. Despite much research effort, the biochemical basis for these clinical connections has yet to be explained. We have identified a structural domain in the C-terminal region of DEK [DEK(309-375)]. DEK(309-375) implies clinical importance because it can reverse the characteristic abnormal DNA-mutagen sensitivity in fibroblasts from ataxia-telangiectasia (A-T) patients. We determined the solution structure of DEK(309-375) by nuclear magnetic resonance spectroscopy, and found it to be structurally homologous to the E2F/DP transcription factor family. On the basis of this homology, we tested whether DEK(309-375) could bind DNA and identified the DNA-interacting surface. DEK presents a hydrophobic surface on the side opposite the DNA-interacting surface. The structure of the C-terminal region of DEK provides insights into the protein function of DEK. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1Q1V is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1Q1V is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q1V OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Devany, M.]] | [[Category: Devany, M.]] | ||
| - | [[Category: Kotharu, N | + | [[Category: Kotharu, N P.]] |
[[Category: Matsuo, H.]] | [[Category: Matsuo, H.]] | ||
[[Category: winged-helix motif]] | [[Category: winged-helix motif]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:34:59 2008'' |
Revision as of 12:35, 21 February 2008
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Structure of the Oncoprotein DEK: a putative DNA-binding Domain Related to the Winged Helix Motif
Contents |
Overview
The chromatin-associated protein DEK was first identified as a fusion protein in patients with a subtype of acute myelogenous leukemia. It has since become associated with diverse human ailments ranging from cancers to autoimmune diseases. Despite much research effort, the biochemical basis for these clinical connections has yet to be explained. We have identified a structural domain in the C-terminal region of DEK [DEK(309-375)]. DEK(309-375) implies clinical importance because it can reverse the characteristic abnormal DNA-mutagen sensitivity in fibroblasts from ataxia-telangiectasia (A-T) patients. We determined the solution structure of DEK(309-375) by nuclear magnetic resonance spectroscopy, and found it to be structurally homologous to the E2F/DP transcription factor family. On the basis of this homology, we tested whether DEK(309-375) could bind DNA and identified the DNA-interacting surface. DEK presents a hydrophobic surface on the side opposite the DNA-interacting surface. The structure of the C-terminal region of DEK provides insights into the protein function of DEK.
Disease
Known disease associated with this structure: Leukemia, acute nonlymphocytic OMIM:[125264]
About this Structure
1Q1V is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Solution NMR structure of the C-terminal domain of the human protein DEK., Devany M, Kotharu NP, Matsuo H, Protein Sci. 2004 Aug;13(8):2252-9. Epub 2004 Jul 6. PMID:15238633
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