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1oyv

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[[Image:1oyv.jpg|left|200px]]
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{{STRUCTURE_1oyv| PDB=1oyv | SCENE= }}
{{STRUCTURE_1oyv| PDB=1oyv | SCENE= }}
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'''Crystal structure of tomato inhibitor-II in a ternary complex with subtilisin Carlsberg'''
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===Crystal structure of tomato inhibitor-II in a ternary complex with subtilisin Carlsberg===
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==Overview==
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Multidomain proteinase inhibitors play critical roles in the defense of plants against predation by a wide range of pests. Despite a wealth of structural information on proteinase-single domain inhibitor interactions, the structural basis of inhibition by multidomain proteinase inhibitors remains poorly understood. Here we report the 2.5-A resolution crystal structure of the two-headed tomato inhibitor-II (TI-II) in complex with two molecules of subtilisin Carlsberg; it reveals how a multidomain inhibitor from the Potato II family of proteinase inhibitors can bind to and simultaneously inhibit two enzyme molecules within a single ternary complex. The N terminus of TI-II initiates the folding of Domain I (Lys-1 to Cys-15 and Pro-84 to Met-123) and then completes Domain II (Ile-26 to Pro-74) before coming back to complete the rest of Domain I (Pro-84 to Met-123). The two domains of TI-II adopt a similar fold and are arranged in an extended configuration that presents two reactive site loops at the opposite ends of the inhibitor molecule. Each subtilisin molecule interacts with a reactive site loop of TI-II through the standard, canonical binding mode. Remarkably, a significant distortion of the active site of subtilisin is induced by the presence of phenylalanine in the P1 position of reactive site loop II of TI-II. The structure of the TI-II.(subtilisin)2 complex provides a molecular framework for understanding how multiple inhibitory domains in a single Potato II type proteinase inhibitor molecule from the Potato II family act to inhibit proteolytic enzymes.
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(as it appears on PubMed at http://www.pubmed.gov), where 12684499 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12684499}}
==About this Structure==
==About this Structure==
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[[Category: Serine proteinase inhibitor]]
[[Category: Serine proteinase inhibitor]]
[[Category: Ternary complex]]
[[Category: Ternary complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:26:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 04:07:20 2008''

Revision as of 01:07, 29 July 2008

Image:1oyv.png

Template:STRUCTURE 1oyv

Crystal structure of tomato inhibitor-II in a ternary complex with subtilisin Carlsberg

Template:ABSTRACT PUBMED 12684499

About this Structure

1OYV is a Protein complex structure of sequences from Bacillus licheniformis and Solanum lycopersicum. Full crystallographic information is available from OCA.

Reference

Structural basis of inhibition revealed by a 1:2 complex of the two-headed tomato inhibitor-II and subtilisin Carlsberg., Barrette-Ng IH, Ng KK, Cherney MM, Pearce G, Ryan CA, James MN, J Biol Chem. 2003 Jun 27;278(26):24062-71. Epub 2003 Apr 8. PMID:12684499

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