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1p0f

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{{STRUCTURE_1p0f| PDB=1p0f | SCENE= }}
{{STRUCTURE_1p0f| PDB=1p0f | SCENE= }}
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'''Crystal Structure of the Binary Complex: NADP(H)-Dependent Vertebrate Alcohol Dehydrogenase (ADH8) with the cofactor NADP'''
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===Crystal Structure of the Binary Complex: NADP(H)-Dependent Vertebrate Alcohol Dehydrogenase (ADH8) with the cofactor NADP===
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==Overview==
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The amphibian enzyme ADH8, previously named class IV-like, is the only known vertebrate alcohol dehydrogenase (ADH) with specificity towards NADP(H). The three-dimensional structures of ADH8 and of the binary complex ADH8-NADP(+) have been now determined and refined to resolutions of 2.2A and 1.8A, respectively. The coenzyme and substrate specificity of ADH8, that has 50-65% sequence identity with vertebrate NAD(H)-dependent ADHs, suggest a role in aldehyde reduction probably as a retinal reductase. The large volume of the substrate-binding pocket can explain both the high catalytic efficiency of ADH8 with retinoids and the high K(m) value for ethanol. Preference of NADP(H) appears to be achieved by the presence in ADH8 of the triad Gly223-Thr224-His225 and the recruitment of conserved Lys228, which define a binding pocket for the terminal phosphate group of the cofactor. NADP(H) binds to ADH8 in an extended conformation that superimposes well with the NAD(H) molecules found in NAD(H)-dependent ADH complexes. No additional reshaping of the dinucleotide-binding site is observed which explains why NAD(H) can also be used as a cofactor by ADH8. The structural features support the classification of ADH8 as an independent ADH class.
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(as it appears on PubMed at http://www.pubmed.gov), where 12818203 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12818203}}
==About this Structure==
==About this Structure==
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[[Category: Valencia, E.]]
[[Category: Valencia, E.]]
[[Category: Adh topology]]
[[Category: Adh topology]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:31:17 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 22:00:12 2008''

Revision as of 19:00, 27 July 2008

Image:1p0f.png

Template:STRUCTURE 1p0f

Crystal Structure of the Binary Complex: NADP(H)-Dependent Vertebrate Alcohol Dehydrogenase (ADH8) with the cofactor NADP

Template:ABSTRACT PUBMED 12818203

About this Structure

1P0F is a Single protein structure of sequence from Rana perezi. Full crystallographic information is available from OCA.

Reference

Crystal structure of the vertebrate NADP(H)-dependent alcohol dehydrogenase (ADH8)., Rosell A, Valencia E, Pares X, Fita I, Farres J, Ochoa WF, J Mol Biol. 2003 Jun 27;330(1):75-85. PMID:12818203

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