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| | {{STRUCTURE_1p20| PDB=1p20 | SCENE= }} | | {{STRUCTURE_1p20| PDB=1p20 | SCENE= }} |
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| - | '''Surprising Roles of Electrostatic Interactions in DNA-Ligand Complexes'''
| + | ===Surprising Roles of Electrostatic Interactions in DNA-Ligand Complexes=== |
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| - | ==Overview==
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| - | The positions of cations in x-ray structures are modulated by sequence, conformation, and ligand interactions. The goal here is to use x-ray diffraction to help resolve structural and thermodynamic roles of specifically localized cations in DNA-anthracycline complexes. We describe a 1.34 A resolution structure of a CGATCG(2)-adriamycin(2) complex obtained from crystals grown in the presence of thallium (I) ions. Tl(+) can substitute for biological monovalent cations, but is readily detected by distinctive x-ray scattering, obviating analysis of subtle differences in coordination geometry and x-ray scattering of water, sodium, potassium, and ammonium. Six localized Tl(+) sites are observable adjacent to each CGATCG(2)-adriamycin(2) complex. Each of these localized monovalent cations are found within the G-tract major groove of the intercalated DNA-drug complex. Adriamycin appears to be designed by nature to interact favorably with the electrostatic landscape of DNA, and to conserve the distribution of localized cationic charge. Localized inorganic cations in the major groove are conserved upon binding of adriamycin. In the minor groove, inorganic cations are substituted by a cationic functional group of adriamycin. This partitioning of cationic charge by adriamycin into the major groove of CG base pairs and the minor groove of AT base pairs may be a general feature of sequence-specific DNA-small molecule interactions and a potentially useful important factor in ligand design. | + | The line below this paragraph, {{ABSTRACT_PUBMED_12717724}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 12717724 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_12717724}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Intercalation]] | | [[Category: Intercalation]] |
| | [[Category: Thallium]] | | [[Category: Thallium]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:34:49 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 04:40:02 2008'' |
Revision as of 01:40, 29 July 2008
Template:STRUCTURE 1p20
Surprising Roles of Electrostatic Interactions in DNA-Ligand Complexes
Template:ABSTRACT PUBMED 12717724
About this Structure
Full crystallographic information is available from OCA.
Reference
Surprising roles of electrostatic interactions in DNA-ligand complexes., Howerton SB, Nagpal A, Williams LD, Biopolymers. 2003 May;69(1):87-99. PMID:12717724
Page seeded by OCA on Tue Jul 29 04:40:02 2008
