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| | {{STRUCTURE_1p6p| PDB=1p6p | SCENE= }} | | {{STRUCTURE_1p6p| PDB=1p6p | SCENE= }} |
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| - | '''Crystal Structure of Toad Liver Basic Fatty Acid-Binding Protein'''
| + | ===Crystal Structure of Toad Liver Basic Fatty Acid-Binding Protein=== |
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| - | ==Overview==
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| - | Two paralogous groups of fatty acid-binding proteins (FABPs) have been described in vertebrate liver: liver FABP (L-FABP) type, extensively characterized in mammals, and liver basic FABP (Lb-FABP) found in fish, amphibians, reptiles, and birds. We describe here the toad Lb-FABP complete amino acid sequence, its X-ray structure to 2.5 A resolution, ligand-binding properties, and mechanism of fatty acid transfer to phospholipid membranes. Alignment of the amino acid sequence of toad Lb-FABP with known L-FABPs and Lb-FABPs shows that it is more closely related to the other Lb-FABPs. Toad Lb-FABP conserves the 12 characteristic residues present in all Lb-FABPs and absent in L-FABPs and presents the canonical fold characteristic of all the members of this protein family. Eight out of the 12 conserved residues point to the lipid-binding cavity of the molecule. In contrast, most of the 25 L-FABP conserved residues are in clusters on the surface of the molecule. The helix-turn-helix motif shows both a negative and positive electrostatic potential surface as in rat L-FABP, and in contrast with the other FABP types. The mechanism of anthroyloxy-labeled fatty acids transfer from Lb-FABP to phospholipid membranes occurs by a diffusion-mediated process, as previously shown for L-FABP, but the rate of transfer is 1 order of magnitude faster. Toad Lb-FABP can bind two cis-parinaric acid molecules but only one trans-parinaric acid molecule while L-FABP binds two molecules of both parinaric acid isomers. Although toad Lb-FABP shares with L-FABP a broad ligand-binding specificity, the relative affinity is different.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_12846568}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 12846568 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_12846568}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Santome, J A.]] | | [[Category: Santome, J A.]] |
| | [[Category: Beta barrel]] | | [[Category: Beta barrel]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:45:17 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:15:59 2008'' |
Revision as of 16:16, 28 July 2008
Template:STRUCTURE 1p6p
Crystal Structure of Toad Liver Basic Fatty Acid-Binding Protein
Template:ABSTRACT PUBMED 12846568
About this Structure
1P6P is a Single protein structure of sequence from Bufo arenarum. Full crystallographic information is available from OCA.
Reference
Structural and biochemical characterization of toad liver fatty acid-binding protein., Di Pietro SM, Corsico B, Perduca M, Monaco HL, Santome JA, Biochemistry. 2003 Jul 15;42(27):8192-203. PMID:12846568
Page seeded by OCA on Mon Jul 28 19:15:59 2008
