1p8k

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{{STRUCTURE_1p8k| PDB=1p8k | SCENE= }}
{{STRUCTURE_1p8k| PDB=1p8k | SCENE= }}
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'''The structure and DNA recognition of a bifunctional homing endonuclease and group I intron splicing factor'''
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===The structure and DNA recognition of a bifunctional homing endonuclease and group I intron splicing factor===
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==Overview==
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We determined the crystal structure of a bifunctional group I intron splicing factor and homing endonuclease, termed the I-AniI maturase, in complex with its DNA target at 2.6 A resolution. The structure demonstrates the remarkable structural conservation of the beta-sheet DNA-binding motif between highly divergent enzyme subfamilies. DNA recognition by I-AniI was further studied using nucleoside deletion and DMS modification interference analyses. Correlation of these results with the crystal structure provides information on the relative importance of individual nucleotide contacts for DNA recognition. Alignment and modeling of two homologous maturases reveals conserved basic surface residues, distant from the DNA-binding surface, that might be involved in RNA binding. A point mutation that introduces a single negative charge in this region uncouples the maturase and endonuclease functions of the protein, inhibiting RNA binding and splicing while maintaining DNA binding and cleavage.
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(as it appears on PubMed at http://www.pubmed.gov), where 14633971 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14633971}}
==About this Structure==
==About this Structure==
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[[Category: Stoddard, B L.]]
[[Category: Stoddard, B L.]]
[[Category: Hydrolase/dna]]
[[Category: Hydrolase/dna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:49:13 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 16:49:15 2008''

Revision as of 13:49, 28 July 2008

Image:1p8k.png

Template:STRUCTURE 1p8k

The structure and DNA recognition of a bifunctional homing endonuclease and group I intron splicing factor

Template:ABSTRACT PUBMED 14633971

About this Structure

1P8K is a Single protein structure of sequence from Emericella nidulans. Full crystallographic information is available from OCA.

Reference

Structural and biochemical analyses of DNA and RNA binding by a bifunctional homing endonuclease and group I intron splicing factor., Bolduc JM, Spiegel PC, Chatterjee P, Brady KL, Downing ME, Caprara MG, Waring RB, Stoddard BL, Genes Dev. 2003 Dec 1;17(23):2875-88. Epub 2003 Nov 21. PMID:14633971

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