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1p9i

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{{STRUCTURE_1p9i| PDB=1p9i | SCENE= }}
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'''Coiled-coil X-ray structure at 1.17 A resolution'''
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===Coiled-coil X-ray structure at 1.17 A resolution===
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==Overview==
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We determined the 1.17 A resolution X-ray crystal structure of a hybrid peptide based on sequences from coiled-coil regions of the proteins GCN4 and cortexillin I. The peptide forms a parallel homodimeric coiled-coil, with C(alpha) backbone geometry similar to GCN4 (rmsd value 0.71 A). Three stabilizing interactions have been identified: a unique hydrogen bonding-electrostatic network not previously observed in coiled-coils, and two other hydrophobic interactions involving leucine residues at positions e and g from both g-a' and d-e' interchain interactions with the hydrophobic core. This is also the first report of the quantitative significance of these interactions. The GCN4/cortexillin hybrid surprisingly has two interchain Glu-Lys' ion pairs that form a hydrogen bonding network with the Asn residues in the core. This network, which was not observed for the reversed Lys-Glu' pair in GCN4, increases the combined stability contribution of each Glu-Lys' salt bridge across the central Asn15-Asn15' core to approximately 0.7 kcal/mole, compared to approximately 0.4 kcal mole(-1) from a Glu-Lys' salt bridge on its own. In addition to electrostatic and hydrogen bonding stabilization of the coiled-coil, individual leucine residues at positions e and g in the hybrid peptide also contribute to stability by 0.7 kcal/mole relative to alanine. These interactions are of critical importance to understanding the stability requirements for coiled-coil folding and in modulating the stability of de novo designed macromolecules containing this motif.
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{{ABSTRACT_PUBMED_12824486}}
==About this Structure==
==About this Structure==
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[[Category: Ivaninskii, S.]]
[[Category: Ivaninskii, S.]]
[[Category: Coiled-coil]]
[[Category: Coiled-coil]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 02:39:48 2008''

Revision as of 23:39, 28 July 2008

Image:1p9i.png

Template:STRUCTURE 1p9i

Coiled-coil X-ray structure at 1.17 A resolution

Template:ABSTRACT PUBMED 12824486

About this Structure

Full crystallographic information is available from OCA.

Reference

Unique stabilizing interactions identified in the two-stranded alpha-helical coiled-coil: crystal structure of a cortexillin I/GCN4 hybrid coiled-coil peptide., Lee DL, Ivaninskii S, Burkhard P, Hodges RS, Protein Sci. 2003 Jul;12(7):1395-405. PMID:12824486

Page seeded by OCA on Tue Jul 29 02:39:48 2008

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