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1r0d
From Proteopedia
(New page: 200px<br /> <applet load="1r0d" size="450" color="white" frame="true" align="right" spinBox="true" caption="1r0d, resolution 1.90Å" /> '''HIP1R THATCH DOMAIN...) |
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| - | [[Image:1r0d.gif|left|200px]]<br /> | + | [[Image:1r0d.gif|left|200px]]<br /><applet load="1r0d" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1r0d" size=" | + | |
caption="1r0d, resolution 1.90Å" /> | caption="1r0d, resolution 1.90Å" /> | ||
'''HIP1R THATCH DOMAIN CORE'''<br /> | '''HIP1R THATCH DOMAIN CORE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Huntingtin-interacting protein-1 related (HIP1R) has a crucial | + | Huntingtin-interacting protein-1 related (HIP1R) has a crucial protein-trafficking role, mediating associations between actin and clathrin-coated structures at the plasma membrane and trans-Golgi network. Here, we characterize the F-actin-binding region of HIP1R, termed the talin-HIP1/R/Sla2p actin-tethering C-terminal homology (THATCH) domain. The 1.9-A crystal structure of the human HIP1R THATCH core reveals a large sequence-conserved surface patch created primarily by residues from the third and fourth helices of a unique five-helix bundle. Point mutations of seven contiguous patch residues produced significant decreases in F-actin binding. We also show that THATCH domains have a conserved C-terminal latch capable of oligomerizing the core, thereby modulating F-actin engagement. Collectively, these results establish a framework for investigating the links between endocytosis and actin dynamics mediated by THATCH domain-containing proteins. |
==About this Structure== | ==About this Structure== | ||
| - | 1R0D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1R0D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R0D OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Brett, T | + | [[Category: Brett, T J.]] |
| - | [[Category: Fremont, D | + | [[Category: Fremont, D H.]] |
| - | [[Category: MCSG, Midwest | + | [[Category: MCSG, Midwest Center for Structural Genomics.]] |
[[Category: actin-binding]] | [[Category: actin-binding]] | ||
[[Category: endocytosis]] | [[Category: endocytosis]] | ||
| Line 25: | Line 24: | ||
[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:45:39 2008'' |
Revision as of 12:45, 21 February 2008
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HIP1R THATCH DOMAIN CORE
Overview
Huntingtin-interacting protein-1 related (HIP1R) has a crucial protein-trafficking role, mediating associations between actin and clathrin-coated structures at the plasma membrane and trans-Golgi network. Here, we characterize the F-actin-binding region of HIP1R, termed the talin-HIP1/R/Sla2p actin-tethering C-terminal homology (THATCH) domain. The 1.9-A crystal structure of the human HIP1R THATCH core reveals a large sequence-conserved surface patch created primarily by residues from the third and fourth helices of a unique five-helix bundle. Point mutations of seven contiguous patch residues produced significant decreases in F-actin binding. We also show that THATCH domains have a conserved C-terminal latch capable of oligomerizing the core, thereby modulating F-actin engagement. Collectively, these results establish a framework for investigating the links between endocytosis and actin dynamics mediated by THATCH domain-containing proteins.
About this Structure
1R0D is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural definition of the F-actin-binding THATCH domain from HIP1R., Brett TJ, Legendre-Guillemin V, McPherson PS, Fremont DH, Nat Struct Mol Biol. 2006 Feb;13(2):121-30. Epub 2006 Jan 15. PMID:16415883
Page seeded by OCA on Thu Feb 21 14:45:39 2008
