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1pci

From Proteopedia

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[[Image:1pci.gif|left|200px]]
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{{STRUCTURE_1pci| PDB=1pci | SCENE= }}
{{STRUCTURE_1pci| PDB=1pci | SCENE= }}
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'''PROCARICAIN'''
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===PROCARICAIN===
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==Overview==
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BACKGROUND: Cysteine proteases are involved in a variety of cellular processes including cartilage degradation in arthritis, the progression of Alzheimer's disease and cancer invasion: these enzymes are therefore of immense biological importance. Caricain is the most basic of the cysteine proteases found in the latex of Carica papaya. It is a member of the papain superfamily and is homologous to other plant and animal cysteine proteases. Caricain is naturally expressed as an inactive zymogen called procaricain. The inactive form of the protease contains an inhibitory proregion which consists of an additional 106 N-terminal amino acids; the proregion is removed upon activation. RESULTS: The crystal structure of procaricain has been refined to 3.2 A resolution; the final model consists of three non-crystallographically related molecules. The proregion of caricain forms a separate globular domain which binds to the C-terminal domain of mature caricain. The proregion also contains an extended polypeptide chain which runs through the substrate-binding cleft, in the opposite direction to that of the substrate, and connects to the N terminus of the mature region. The mature region does not undergo any conformational change on activation. CONCLUSIONS: We conclude that the rate-limiting step in the in vitro activation of procaricain is the dissociation of the prodomain, which is then followed by proteolytic cleavage of the extended polypeptide chain of the proregion. The prodomain provides a stable scaffold which may facilitate the folding of the C-terminal lobe of procaricain.
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(as it appears on PubMed at http://www.pubmed.gov), where 8939744 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8939744}}
==About this Structure==
==About this Structure==
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[[Category: Thiol protease]]
[[Category: Thiol protease]]
[[Category: Zymogen]]
[[Category: Zymogen]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:56:10 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:05:36 2008''

Revision as of 20:05, 27 July 2008

Image:1pci.png

Template:STRUCTURE 1pci

PROCARICAIN

Template:ABSTRACT PUBMED 8939744

About this Structure

1PCI is a Single protein structure of sequence from Carica papaya. Full crystallographic information is available from OCA.

Reference

The prosequence of procaricain forms an alpha-helical domain that prevents access to the substrate-binding cleft., Groves MR, Taylor MA, Scott M, Cummings NJ, Pickersgill RW, Jenkins JA, Structure. 1996 Oct 15;4(10):1193-203. PMID:8939744

Page seeded by OCA on Sun Jul 27 23:05:36 2008

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