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1pey

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[[Image:1pey.gif|left|200px]]
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{{STRUCTURE_1pey| PDB=1pey | SCENE= }}
{{STRUCTURE_1pey| PDB=1pey | SCENE= }}
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'''Crystal structure of the Response Regulator Spo0F complexed with Mn2+'''
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===Crystal structure of the Response Regulator Spo0F complexed with Mn2+===
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==Overview==
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As a part of studies on the structural characterization of the components of the sporulation phosphorelay in Bacillus subtilis, the crystal structure of the manganese derivative of an intermediate signal transducer, Spo0F, has been elucidated at 2.25 A resolution. The calcium complex and the apo structures have been analyzed previously. In apo Spo0F, the active-site cation cavity is only partially formed and it only becomes completed upon metal coordination. The carbonyl of Lys56 is coordinated to the metal and interestingly the side chain of Lys56 exists in a variety of conformations in the three crystal structures of Spo0F. The affinity of the magnesium ion for Spo0F is in fact low; however, it binds Spo0F when it is in complex with Spo0B. It is proposed that the existence of a deep pocket which extends from the surface to the metal site could attract and direct the metal, thereby facilitating the metal binding of the complex.
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(as it appears on PubMed at http://www.pubmed.gov), where 15039551 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15039551}}
==About this Structure==
==About this Structure==
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[[Category: Zapf, J.]]
[[Category: Zapf, J.]]
[[Category: Cation binding]]
[[Category: Cation binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:00:43 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:54:17 2008''

Revision as of 14:54, 27 July 2008

Image:1pey.png

Template:STRUCTURE 1pey

Crystal structure of the Response Regulator Spo0F complexed with Mn2+

Template:ABSTRACT PUBMED 15039551

About this Structure

1PEY is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

Metals in the sporulation phosphorelay: manganese binding by the response regulator Spo0F., Mukhopadhyay D, Sen U, Zapf J, Varughese KI, Acta Crystallogr D Biol Crystallogr. 2004 Apr;60(Pt 4):638-45. Epub 2004, Mar 23. PMID:15039551

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