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1pfs

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{{STRUCTURE_1pfs| PDB=1pfs | SCENE= }}
{{STRUCTURE_1pfs| PDB=1pfs | SCENE= }}
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'''SOLUTION NMR STRUCTURE OF THE SINGLE-STRANDED DNA BINDING PROTEIN OF THE FILAMENTOUS PSEUDOMONAS PHAGE PF3, MINIMIZED AVERAGE STRUCTURE'''
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===SOLUTION NMR STRUCTURE OF THE SINGLE-STRANDED DNA BINDING PROTEIN OF THE FILAMENTOUS PSEUDOMONAS PHAGE PF3, MINIMIZED AVERAGE STRUCTURE===
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==Overview==
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The three-dimensional structure of the homodimeric single-stranded DNA binding protein encoded by the filamentous Pseudomonas bacteriophage Pf3 has been determined using heteronuclear multidimensional NMR techniques and restrained molecular dynamics. NMR experiments and structure calculations have been performed on a mutant protein (Phe36 --&gt; His) that was successfully designed to reduce the tendency of the protein to aggregate. The protein monomer is composed of a five-stranded antiparallel beta-sheet from which two beta-hairpins and a large loop protrude. The structure is compared with the single-stranded DNA binding protein encoded by the filamentous Escherichia coli phage Ff, a protein with a similar biological function and DNA binding properties, yet quite different amino acid sequence, and with the major cold shock protein of Escherichia coli, a single-stranded DNA binding protein with an entirely different sequence, biological function and binding characteristics. The amino acid sequence of the latter is highly homologous to the nucleic acid binding domain (i.e. the cold shock domain) of proteins belonging to the Y-box family. Despite their differences in amino acid sequence and function, the folds of the three proteins are remarkably similar, suggesting that this is a preferred folding pattern shared by many single-stranded DNA binding proteins.
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{{ABSTRACT_PUBMED_7556054}}
==About this Structure==
==About this Structure==
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1PFS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_pf3 Pseudomonas phage pf3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PFS OCA].
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1PFS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_pf3 Pseudomonas phage pf3]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PFS OCA].
==Reference==
==Reference==
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[[Category: Single-stranded dna]]
[[Category: Single-stranded dna]]
[[Category: Viral]]
[[Category: Viral]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:02:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 17:48:20 2008''

Revision as of 14:48, 27 July 2008

Image:1pfs.png

Template:STRUCTURE 1pfs

SOLUTION NMR STRUCTURE OF THE SINGLE-STRANDED DNA BINDING PROTEIN OF THE FILAMENTOUS PSEUDOMONAS PHAGE PF3, MINIMIZED AVERAGE STRUCTURE

Template:ABSTRACT PUBMED 7556054

About this Structure

1PFS is a Single protein structure of sequence from Pseudomonas phage pf3. Full experimental information is available from OCA.

Reference

Solution structure of the single-stranded DNA binding protein of the filamentous Pseudomonas phage Pf3: similarity to other proteins binding to single-stranded nucleic acids., Folmer RH, Nilges M, Konings RN, Hilbers CW, EMBO J. 1995 Sep 1;14(17):4132-42. PMID:7556054

Page seeded by OCA on Sun Jul 27 17:48:20 2008

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