1r4n
From Proteopedia
(New page: 200px<br /> <applet load="1r4n" size="450" color="white" frame="true" align="right" spinBox="true" caption="1r4n, resolution 3.6Å" /> '''APPBP1-UBA3-NEDD8, a...) |
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| - | [[Image:1r4n. | + | [[Image:1r4n.jpg|left|200px]]<br /><applet load="1r4n" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1r4n" size=" | + | |
caption="1r4n, resolution 3.6Å" /> | caption="1r4n, resolution 3.6Å" /> | ||
'''APPBP1-UBA3-NEDD8, an E1-ubiquitin-like protein complex with ATP'''<br /> | '''APPBP1-UBA3-NEDD8, an E1-ubiquitin-like protein complex with ATP'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1R4N is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ZN and ATP as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1R4N with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb60_1.html Ubiquitin]]. Full crystallographic information is available from [http:// | + | 1R4N is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=ATP:'>ATP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1R4N with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb60_1.html Ubiquitin]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R4N OCA]. |
==Reference== | ==Reference== | ||
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[[Category: cell cycle]] | [[Category: cell cycle]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:46:52 2008'' |
Revision as of 14:46, 15 February 2008
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APPBP1-UBA3-NEDD8, an E1-ubiquitin-like protein complex with ATP
Overview
E1 enzymes initiate ubiquitin-like protein (ubl) transfer cascades by, catalyzing adenylation of the ubl's C terminus. An E1's selectivity for, its cognate ubl is essential because the E1 subsequently coordinates the, ubl with its correct downstream pathway. We report here the structure of, the 120 kDa quaternary complex between human APPBP1-UBA3, a heterodimeric, E1, its ubl NEDD8, and ATP. The E1 selectively recruits NEDD8 through a, bipartite interface, involving a domain common to all ubl activating, enzymes including bacterial ancestors, and also eukaryotic E1-specific, sequences. By modeling ubiquitin into the NEDD8 binding site and, performing mutational analysis, we identify a single conserved arginine in, APPBP1-UBA3 that acts as a selectivity gate, preventing misactivation of, ubiquitin by NEDD8's E1. NEDD8 residues that interact with E1 correspond, to residues in ubiquitin important for binding the proteasome and other, ubiquitin-interacting proteins, suggesting that the conjugation and, recognition machineries have coevolved for each specific ubl.
About this Structure
1R4N is a Protein complex structure of sequences from Homo sapiens with and as ligands. The following page contains interesting information on the relation of 1R4N with [Ubiquitin]. Full crystallographic information is available from OCA.
Reference
The structure of the APPBP1-UBA3-NEDD8-ATP complex reveals the basis for selective ubiquitin-like protein activation by an E1., Walden H, Podgorski MS, Huang DT, Miller DW, Howard RJ, Minor DL Jr, Holton JM, Schulman BA, Mol Cell. 2003 Dec;12(6):1427-37. PMID:14690597
Page seeded by OCA on Fri Feb 15 16:46:52 2008
Categories: Homo sapiens | Protein complex | Ubiquitin | Holton, J.M | Podgorski, M.S. | Schulman, B.A. | Walden, H. | ATP | ZN | Cell cycle
