This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1pjy

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1pjy.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1pjy.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1pjy| PDB=1pjy | SCENE= }}
{{STRUCTURE_1pjy| PDB=1pjy | SCENE= }}
-
'''Solution structure of the HIV-1 frameshift inducing stem-loop RNA'''
+
===Solution structure of the HIV-1 frameshift inducing stem-loop RNA===
-
==Overview==
+
<!--
-
The translation of reverse transcriptase and other essential viral proteins from the HIV-1 Pol mRNA requires a programmed -1 ribosomal frameshift. This frameshift is induced by two highly conserved elements within the HIV-1 mRNA: a slippery sequence comprised of a UUUUUUA heptamer, and a downstream stem-loop structure. We have determined the structure of the HIV-1 frameshift inducing RNA stem-loop, using multidimensional heteronuclear nuclear magnetic resonance (NMR) methods. The 22 nucleotide RNA solution structure [root mean squared deviation (r.m.s.d.) = 1.2 A] was determined from 475 nuclear Overhauser effect (NOE)-derived distance restrains, 20 residual dipolar couplings and direct detection of hydrogen bonds via scalar couplings. We find that the frameshift inducing stem-loop is an A-form helix capped by a structured ACAA tetraloop. The ACAA tetraloop is stabilized by an equilateral 5' and 3' stacking pattern, a sheared A-A pair and a cross-strand hydrogen bond. Unexpectedly, the ACAA tetraloop structure is nearly identical to a known tetraloop fold, previously identified in the RNase III recognition site from Saccharomyces cerevisiae.
+
The line below this paragraph, {{ABSTRACT_PUBMED_12888491}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 12888491 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_12888491}}
==About this Structure==
==About this Structure==
-
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJY OCA].
+
Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJY OCA].
==Reference==
==Reference==
Line 27: Line 31:
[[Category: Rna structure]]
[[Category: Rna structure]]
[[Category: Tetraloop]]
[[Category: Tetraloop]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:10:17 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:52:21 2008''

Revision as of 01:52, 28 July 2008

Image:1pjy.png

Template:STRUCTURE 1pjy

Solution structure of the HIV-1 frameshift inducing stem-loop RNA

Template:ABSTRACT PUBMED 12888491

About this Structure

Full experimental information is available from OCA.

Reference

Solution structure of the HIV-1 frameshift inducing stem-loop RNA., Staple DW, Butcher SE, Nucleic Acids Res. 2003 Aug 1;31(15):4326-31. PMID:12888491

Page seeded by OCA on Mon Jul 28 04:52:21 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1pjy"
Personal tools