1pk5

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1pk5.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1pk5.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1pk5| PDB=1pk5 | SCENE= }}
{{STRUCTURE_1pk5| PDB=1pk5 | SCENE= }}
-
'''Crystal structure of the orphan nuclear receptor LRH-1'''
+
===Crystal structure of the orphan nuclear receptor LRH-1===
-
==Overview==
+
<!--
-
The orphan nuclear receptors SF-1 and LRH-1 are constitutively active, but it remains uncertain whether their activation is hormone dependent. We report the crystal structure of the LRH-1 ligand binding domain to 2.4 A resolution and find the receptor to be a monomer that adopts an active conformation with a large but empty hydrophobic pocket. Adding bulky side chains into this pocket resulted in full or greater activity suggesting that, while LRH-1 could accommodate potential ligands, these are dispensable for basal activity. Constitutive LRH-1 activity appears to be conferred by a distinct structural element consisting of an extended helix 2 that provides an additional layer to the canonical LBD fold. Mutating the conserved arginine in helix 2 reduced LRH-1 receptor activity and coregulator recruitment, consistent with the partial loss-of-function phenotype exhibited by an analogous SF-1 human mutant. These findings illustrate an alternative structural strategy for nuclear receptor stabilization in the absence of ligand binding.
+
The line below this paragraph, {{ABSTRACT_PUBMED_12820970}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 12820970 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_12820970}}
==About this Structure==
==About this Structure==
Line 29: Line 33:
[[Category: Lrh-1]]
[[Category: Lrh-1]]
[[Category: Nuclear receptor]]
[[Category: Nuclear receptor]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:10:52 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:40:13 2008''

Revision as of 12:40, 29 July 2008

Image:1pk5.png

Template:STRUCTURE 1pk5

Crystal structure of the orphan nuclear receptor LRH-1

Template:ABSTRACT PUBMED 12820970

About this Structure

1PK5 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

Structural basis for ligand-independent activation of the orphan nuclear receptor LRH-1., Sablin EP, Krylova IN, Fletterick RJ, Ingraham HA, Mol Cell. 2003 Jun;11(6):1575-85. PMID:12820970

Page seeded by OCA on Tue Jul 29 15:40:13 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1pk5"
Personal tools