1pk8

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1pk8.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1pk8.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1pk8| PDB=1pk8 | SCENE= }}
{{STRUCTURE_1pk8| PDB=1pk8 | SCENE= }}
-
'''Crystal Structure of Rat Synapsin I C Domain Complexed to Ca.ATP'''
+
===Crystal Structure of Rat Synapsin I C Domain Complexed to Ca.ATP===
-
==Overview==
+
<!--
-
Synapsins are multidomain proteins that are critical for regulating neurotransmitter release in vertebrates. In the present study, two crystal structures of the C domain of rat synapsin I (rSynI-C) in complex with Ca(2+) and ATP reveal that this protein can form a tetramer and that a flexible loop (the "multifunctional loop") contacts bound ATP. Further experiments were carried out on a protein comprising the A, B, and C domains of rat synapsin I (rSynI-ABC). An ATP-stabilized tetramer of rSynI-ABC is observed during velocity sedimentation and size-exclusion chromatographic experiments. These hydrodynamic results also indicate that the A and B domains exist in an extended conformation. Calorimetric measurements of ATP binding to wild-type and mutant rSynI-ABC demonstrate that the multifunctional loop and a cross-tetramer contact are important for ATP binding. The evidence supports a view of synapsin I as an ATP-utilizing, tetrameric protein made up of monomers that have a flexible, extended N terminus.
+
The line below this paragraph, {{ABSTRACT_PUBMED_14688264}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 14688264 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_14688264}}
==About this Structure==
==About this Structure==
Line 27: Line 31:
[[Category: Atp binding]]
[[Category: Atp binding]]
[[Category: Atp grasp]]
[[Category: Atp grasp]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:10:57 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:57:25 2008''

Revision as of 02:57, 29 July 2008

Image:1pk8.png

Template:STRUCTURE 1pk8

Crystal Structure of Rat Synapsin I C Domain Complexed to Ca.ATP

Template:ABSTRACT PUBMED 14688264

About this Structure

1PK8 is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Tetramerization and ATP binding by a protein comprising the A, B, and C domains of rat synapsin I., Brautigam CA, Chelliah Y, Deisenhofer J, J Biol Chem. 2004 Mar 19;279(12):11948-56. Epub 2003 Dec 19. PMID:14688264

Page seeded by OCA on Tue Jul 29 05:57:25 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1pk8"
Personal tools