8v44
From Proteopedia
(Difference between revisions)
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8v44 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8v44 OCA], [https://pdbe.org/8v44 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8v44 RCSB], [https://www.ebi.ac.uk/pdbsum/8v44 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8v44 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8v44 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8v44 OCA], [https://pdbe.org/8v44 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8v44 RCSB], [https://www.ebi.ac.uk/pdbsum/8v44 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8v44 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0AA82WPF0_PSEAI A0AA82WPF0_PSEAI] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | CasDinG is an ATP-dependent 5'-3' DNA helicase essential for bacterial Type IV-A1 CRISPR associated immunity. CasDinG contains an essential N-terminal domain predicted to bind DNA. To better understand the role of the N-terminal domain, we attempted to co-crystallize CasDinG with DNA substrates. We successfully crystallized CasDinG in a tightly packed, crystal conformation with previously unobserved unit cell dimensions. However, the structure lacked electron density for a bound DNA substrate and the CasDinG N-terminal domain. Additionally, the tight crystal packing disallowed space for the N-terminal domain, indicating that the N-terminal domain was proteolyzed before crystallization. Follow up experiments revealed that the N-terminal domain of CasDinG is proteolyzed after a few days at room temperature, but is protected from proteolysis at 4 degrees C. These data provide a distinct x-ray crystal structure of CasDinG and indicate the essential N-terminal domain of CasDinG is prone to proteolysis. | ||
| + | |||
| + | The N-terminal domain of Type IV-A1 CRISPR-associated DinG is vulnerable to proteolysis.,Hallmark T, Williams AA, Redman O, Guinn B, Judd C, Jackson RN MicroPubl Biol. 2024 Jun 5;2024:10.17912/micropub.biology.001226. doi: , 10.17912/micropub.biology.001226. eCollection 2024. PMID:38911435<ref>PMID:38911435</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 8v44" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
N-terminal truncation of CRISPR-associated DinG
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