1pn8

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{{STRUCTURE_1pn8| PDB=1pn8 | SCENE= }}
{{STRUCTURE_1pn8| PDB=1pn8 | SCENE= }}
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'''Coordinates of S12, L11 proteins and E-site tRNA from 70S crystal structure separately fitted into the Cryo-EM map of E.coli 70S.EF-G.GDPNP complex. The atomic coordinates originally from the E-site tRNA were fitted in the position of the hybrid P/E-site tRNA.'''
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===Coordinates of S12, L11 proteins and E-site tRNA from 70S crystal structure separately fitted into the Cryo-EM map of E.coli 70S.EF-G.GDPNP complex. The atomic coordinates originally from the E-site tRNA were fitted in the position of the hybrid P/E-site tRNA.===
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==Overview==
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During the ribosomal translocation, the binding of elongation factor G (EF-G) to the pretranslocational ribosome leads to a ratchet-like rotation of the 30S subunit relative to the 50S subunit in the direction of the mRNA movement. By means of cryo-electron microscopy we observe that this rotation is accompanied by a 20 A movement of the L1 stalk of the 50S subunit, implying that this region is involved in the translocation of deacylated tRNAs from the P to the E site. These ribosomal motions can occur only when the P-site tRNA is deacylated. Prior to peptidyl-transfer to the A-site tRNA or peptide removal, the presence of the charged P-site tRNA locks the ribosome and prohibits both of these motions.
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(as it appears on PubMed at http://www.pubmed.gov), where 12859903 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12859903}}
==About this Structure==
==About this Structure==
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[[Category: Trna]]
[[Category: Trna]]
[[Category: Trna binding protein]]
[[Category: Trna binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:16:23 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:02:23 2008''

Revision as of 11:02, 29 July 2008

Image:1pn8.png

Template:STRUCTURE 1pn8

Coordinates of S12, L11 proteins and E-site tRNA from 70S crystal structure separately fitted into the Cryo-EM map of E.coli 70S.EF-G.GDPNP complex. The atomic coordinates originally from the E-site tRNA were fitted in the position of the hybrid P/E-site tRNA.

Template:ABSTRACT PUBMED 12859903

About this Structure

1PN8 is a Protein complex structure of sequences from Thermotoga maritima and Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Locking and unlocking of ribosomal motions., Valle M, Zavialov A, Sengupta J, Rawat U, Ehrenberg M, Frank J, Cell. 2003 Jul 11;114(1):123-34. PMID:12859903

Page seeded by OCA on Tue Jul 29 14:02:23 2008

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