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| - | [[Image:1po3.jpg|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1po3| PDB=1po3 | SCENE= }} | | {{STRUCTURE_1po3| PDB=1po3 | SCENE= }} |
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| - | '''Crystal structure of ferric citrate transporter FecA in complex with ferric citrate'''
| + | ===Crystal structure of ferric citrate transporter FecA in complex with ferric citrate=== |
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| - | ==Overview==
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| - | Escherichia coli possesses a TonB-dependent transport system, which exploits the iron-binding capacity of citrate and its natural abundance. Here, we describe three structures of the outer membrane ferric citrate transporter FecA: unliganded and complexed with iron-free or diferric dicitrate. We show the structural mechanism for discrimination between the iron-free and ferric siderophore: the binding of diferric dicitrate, but not iron-free dicitrate alone, causes major conformational rearrangements in the transporter. The structure of FecA bound with iron-free dicitrate represents the first structure of a TonB-dependent transporter bound with an iron-free siderophore. Binding of diferric dicitrate to FecA results in changes in the orientation of the two citrate ions relative to each other and in their interactions with FecA, compared to the binding of iron-free dicitrate. The changes in ligand binding are accompanied by conformational changes in three areas of FecA: two extracellular loops, one plug domain loop and the periplasmic TonB-box motif. The positional and conformational changes in the siderophore and transporter initiate two independent events: ferric citrate transport into the periplasm and transcription induction of the fecABCDE transport genes. From these data, we propose a two-step ligand recognition event: FecA binds iron-free dicitrate in the non-productive state or first step, followed by siderophore displacement to form the transport-competent, diferric dicitrate-bound state in the second step.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_12948487}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 12948487 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_12948487}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Siderophore]] | | [[Category: Siderophore]] |
| | [[Category: Tonb-dependent transport]] | | [[Category: Tonb-dependent transport]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:17:54 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:02:26 2008'' |
Revision as of 12:02, 27 July 2008
Template:STRUCTURE 1po3
Crystal structure of ferric citrate transporter FecA in complex with ferric citrate
Template:ABSTRACT PUBMED 12948487
About this Structure
1PO3 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structural evidence for iron-free citrate and ferric citrate binding to the TonB-dependent outer membrane transporter FecA., Yue WW, Grizot S, Buchanan SK, J Mol Biol. 2003 Sep 12;332(2):353-68. PMID:12948487
Page seeded by OCA on Sun Jul 27 15:02:26 2008
