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1poa

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[[Image:1poa.gif|left|200px]]
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{{STRUCTURE_1poa| PDB=1poa | SCENE= }}
{{STRUCTURE_1poa| PDB=1poa | SCENE= }}
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'''INTERFACIAL CATALYSIS: THE MECHANISM OF PHOSPHOLIPASE A2'''
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===INTERFACIAL CATALYSIS: THE MECHANISM OF PHOSPHOLIPASE A2===
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==Overview==
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A chemical description of the action of phospholipase A2 (PLA2) can now be inferred with confidence from three high-resolution x-ray crystal structures. The first is the structure of the PLA2 from the venom of the Chinese cobra (Naja naja atra) in a complex with a phosphonate transition-state analogue. This enzyme is typical of a large, well-studied homologous family of PLA2S. The second is a similar complex with the evolutionarily distant bee-venom PLA2. The third structure is the uninhibited PLA2 from Chinese cobra venom. Despite the different molecular architectures of the cobra and bee-venom PLA2s, the transition-state analogue interacts in a nearly identical way with the catalytic machinery of both enzymes. The disposition of the fatty-acid side chains suggests a common access route of the substrate from its position in the lipid aggregate to its productive interaction with the active site. Comparison of the cobra-venom complex with the uninhibited enzyme indicates that optimal binding and catalysis at the lipid-water interface is due to facilitated substrate diffusion from the interfacial binding surface to the catalytic site rather than an allosteric change in the enzyme's structure. However, a second bound calcium ion changes its position upon the binding of the transition-state analogue, suggesting a mechanism for augmenting the critical electrophile.
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The line below this paragraph, {{ABSTRACT_PUBMED_2274785}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 2274785 is the PubMed ID number.
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{{ABSTRACT_PUBMED_2274785}}
==About this Structure==
==About this Structure==
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[[Category: Sigler, P B.]]
[[Category: Sigler, P B.]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:18:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:19:51 2008''

Revision as of 11:19, 27 July 2008

Image:1poa.png

Template:STRUCTURE 1poa

INTERFACIAL CATALYSIS: THE MECHANISM OF PHOSPHOLIPASE A2

Template:ABSTRACT PUBMED 2274785

About this Structure

1POA is a Single protein structure of sequence from Naja atra. Full crystallographic information is available from OCA.

Reference

Interfacial catalysis: the mechanism of phospholipase A2., Scott DL, White SP, Otwinowski Z, Yuan W, Gelb MH, Sigler PB, Science. 1990 Dec 14;250(4987):1541-6. PMID:2274785

Page seeded by OCA on Sun Jul 27 14:19:51 2008

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