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1pqh

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{{STRUCTURE_1pqh| PDB=1pqh | SCENE= }}
{{STRUCTURE_1pqh| PDB=1pqh | SCENE= }}
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'''Serine 25 to Threonine mutation of aspartate decarboxylase'''
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===Serine 25 to Threonine mutation of aspartate decarboxylase===
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==Overview==
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Aspartate decarboxylase, which is translated as a pro-protein, undergoes intramolecular self-cleavage at Gly24-Ser25. We have determined the crystal structures of an unprocessed native precursor, in addition to Ala24 insertion, Ala26 insertion and Gly24--&gt;Ser, His11--&gt;Ala, Ser25--&gt;Ala, Ser25--&gt;Cys and Ser25--&gt;Thr mutants. Comparative analyses of the cleavage site reveal specific conformational constraints that govern self-processing and demonstrate that considerable rearrangement must occur. We suggest that Thr57 Ogamma and a water molecule form an 'oxyanion hole' that likely stabilizes the proposed oxyoxazolidine intermediate. Thr57 and this water molecule are probable catalytic residues able to support acid-base catalysis. The conformational freedom in the loop preceding the cleavage site appears to play a determining role in the reaction. The molecular mechanism of self-processing, presented here, emphasizes the importance of stabilization of the oxyoxazolidine intermediate. Comparison of the structural features shows significant similarity to those in other self-processing systems, and suggests that models of the cleavage site of such enzymes based on Ser--&gt;Ala or Ser--&gt;Thr mutants alone may lead to erroneous interpretations of the mechanism.
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{{ABSTRACT_PUBMED_14633979}}
==About this Structure==
==About this Structure==
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[[Category: Protein self-processing]]
[[Category: Protein self-processing]]
[[Category: Pyruvoyl dependent decarboxylase]]
[[Category: Pyruvoyl dependent decarboxylase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:22:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 04:55:51 2008''

Revision as of 01:55, 29 July 2008

Image:1pqh.png

Template:STRUCTURE 1pqh

Serine 25 to Threonine mutation of aspartate decarboxylase

Template:ABSTRACT PUBMED 14633979

About this Structure

1PQH is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structural constraints on protein self-processing in L-aspartate-alpha-decarboxylase., Schmitzberger F, Kilkenny ML, Lobley CM, Webb ME, Vinkovic M, Matak-Vinkovic D, Witty M, Chirgadze DY, Smith AG, Abell C, Blundell TL, EMBO J. 2003 Dec 1;22(23):6193-204. PMID:14633979

Page seeded by OCA on Tue Jul 29 04:55:51 2008

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