1pqt

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{{Seed}}
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{{STRUCTURE_1pqt| PDB=1pqt | SCENE= }}
{{STRUCTURE_1pqt| PDB=1pqt | SCENE= }}
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'''REFINEMENT OF d(GCGAAGC) HAIRPIN STRUCTURE USING ONE- AND TWO-BOND RESIDUAL DIPOLAR COUPLINGS'''
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===REFINEMENT OF d(GCGAAGC) HAIRPIN STRUCTURE USING ONE- AND TWO-BOND RESIDUAL DIPOLAR COUPLINGS===
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==Overview==
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The structure of the 13C,15N-labeled d(GCGAAGC) hairpin, as determined by NMR spectroscopy and refined using molecular dynamics with NOE-derived distances, torsion angles, and residual dipolar couplings (RDCs), is presented. Although the studied molecule is of small size, it is demonstrated that the incorporation of diminutive RDCs can significantly improve local structure determination of regions undefined by the conventional restraints. Very good correlation between the experimental and back-calculated small one- and two-bond 1H-13C, 1H-15N, 13C-13C and 13C-15N coupling constants has been attained. The final structures clearly show typical features of the miniloop architecture. The structure is discussed in context of the extraordinary stability of the d(GCGAAGC) hairpin, which originates from a complex interplay between the aromatic base stacking and hydrogen bonding interactions.
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(as it appears on PubMed at http://www.pubmed.gov), where 12449414 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12449414}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PQT OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PQT OCA].
==Reference==
==Reference==
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[[Category: Ga mismatch]]
[[Category: Ga mismatch]]
[[Category: Hairpin]]
[[Category: Hairpin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:23:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:59:55 2008''

Revision as of 12:59, 27 July 2008

Image:1pqt.png

Template:STRUCTURE 1pqt

REFINEMENT OF d(GCGAAGC) HAIRPIN STRUCTURE USING ONE- AND TWO-BOND RESIDUAL DIPOLAR COUPLINGS

Template:ABSTRACT PUBMED 12449414

About this Structure

Full experimental information is available from OCA.

Reference

Refinement of d(GCGAAGC) hairpin structure using one- and two-bond residual dipolar couplings., Padrta P, Stefl R, Kralik L, Zidek L, Sklenar V, J Biomol NMR. 2002 Sep;24(1):1-14. PMID:12449414

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