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1pru

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[[Image:1pru.jpg|left|200px]]
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{{STRUCTURE_1pru| PDB=1pru | SCENE= }}
{{STRUCTURE_1pru| PDB=1pru | SCENE= }}
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'''PURINE REPRESSOR DNA-BINDING DOMAIN DNA BINDING'''
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===PURINE REPRESSOR DNA-BINDING DOMAIN DNA BINDING===
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==Overview==
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BACKGROUND: The purine repressor (PurR) regulates genes that encode enzymes for purine biosynthesis. PurR has a two domain structure with an N-terminal DNA-binding domain (DBD) and a C-terminal corepressor-binding domain (CBD). The three dimensional structure of a ternary complex of PurR bound to both corepressor and a specific DNA sequence has recently been determined by X-ray crystallography. RESULTS: We have determined the solution structure of the PurR DBD by NMR. It contains three helices, with the first and second helices forming a helix-turn-helix motif. The tertiary structure of the three helices is very similar to that of the corresponding region in the ternary complex. The structure of the hinge helical region, however, which makes specific base contacts in the minor groove of DNA, is disordered in the DNA-free form. CONCLUSION: The stable formation of PurR hinge helices requires PurR dimerization, which brings the hinge regions proximal to each other. The dimerization of the hinge helices is likely to be controlled by the CBD dimerization interface, but is induced by specific-DNA binding.
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(as it appears on PubMed at http://www.pubmed.gov), where 8591032 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8591032}}
==About this Structure==
==About this Structure==
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1PRU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PRU OCA].
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1PRU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PRU OCA].
==Reference==
==Reference==
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[[Category: Yamamoto, H.]]
[[Category: Yamamoto, H.]]
[[Category: Purine repressor]]
[[Category: Purine repressor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:24:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:28:50 2008''

Revision as of 12:28, 29 July 2008

Image:1pru.png

Template:STRUCTURE 1pru

PURINE REPRESSOR DNA-BINDING DOMAIN DNA BINDING

Template:ABSTRACT PUBMED 8591032

About this Structure

1PRU is a Single protein structure of sequence from Escherichia coli. Full experimental information is available from OCA.

Reference

Structural comparison of the free and DNA-bound forms of the purine repressor DNA-binding domain., Nagadoi A, Morikawa S, Nakamura H, Enari M, Kobayashi K, Yamamoto H, Sampei G, Mizobuchi K, Schumacher MA, Brennan RG, et al., Structure. 1995 Nov 15;3(11):1217-24. PMID:8591032

Page seeded by OCA on Tue Jul 29 15:28:50 2008

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