1pt2

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{{STRUCTURE_1pt2| PDB=1pt2 | SCENE= }}
{{STRUCTURE_1pt2| PDB=1pt2 | SCENE= }}
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'''Crystal structure of levansucrase (E342A) complexed with sucrose'''
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===Crystal structure of levansucrase (E342A) complexed with sucrose===
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==Overview==
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Many bacteria and about 40,000 plant species form primary carbohydrate reserves based on fructan; these polymers of beta-D-fructofuranose are thought to confer tolerance to drought and frost in plants. Microbial fructan, the beta(2,6)-linked levan, is synthesized directly from sucrose by levansucrase, which is able to catalyze both sucrose hydrolysis and levan polymerization. The crystal structure of Bacillus subtilis levansucrase, determined to a resolution of 1.5 A, shows a rare five-fold beta-propeller topology with a deep, negatively charged central pocket. Arg360, a residue essential for polymerase activity, lies in a solvent-exposed site adjacent to the central pocket. Mutagenesis data and the sucrose-bound structure of inactive levansucrase E342A, at a resolution of 2.1 A, strongly suggest that three conserved acidic side chains in the central pocket are critical for catalysis, and presumably function as nucleophile (Asp86) and general acid (Glu342), or stabilize the transition state (Asp247).
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(as it appears on PubMed at http://www.pubmed.gov), where 14517548 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14517548}}
==About this Structure==
==About this Structure==
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[[Category: Levansucrase]]
[[Category: Levansucrase]]
[[Category: Sucrose]]
[[Category: Sucrose]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:27:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:24:56 2008''

Revision as of 02:25, 28 July 2008

Image:1pt2.png

Template:STRUCTURE 1pt2

Crystal structure of levansucrase (E342A) complexed with sucrose

Template:ABSTRACT PUBMED 14517548

About this Structure

1PT2 is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

Structural framework of fructosyl transfer in Bacillus subtilis levansucrase., Meng G, Futterer K, Nat Struct Biol. 2003 Nov;10(11):935-41. Epub 2003 Sep 28. PMID:14517548

Page seeded by OCA on Mon Jul 28 05:24:56 2008

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