1ajs
From Proteopedia
(New page: 200px<br /> <applet load="1ajs" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ajs, resolution 1.60Å" /> '''REFINEMENT AND COMP...) |
|||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1AJS is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]] with PLA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.1 2.6.1.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AJS OCA]]. | + | 1AJS is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]] with PLA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Aspartate_transaminase Aspartate transaminase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.1 2.6.1.1]]. Structure known Active Sites: ACT and AXT. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AJS OCA]]. |
==Reference== | ==Reference== | ||
Refinement and comparisons of the crystal structures of pig cytosolic aspartate aminotransferase and its complex with 2-methylaspartate., Rhee S, Silva MM, Hyde CC, Rogers PH, Metzler CM, Metzler DE, Arnone A, J Biol Chem. 1997 Jul 11;272(28):17293-302. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9211866 9211866] | Refinement and comparisons of the crystal structures of pig cytosolic aspartate aminotransferase and its complex with 2-methylaspartate., Rhee S, Silva MM, Hyde CC, Rogers PH, Metzler CM, Metzler DE, Arnone A, J Biol Chem. 1997 Jul 11;272(28):17293-302. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9211866 9211866] | ||
| + | [[Category: Aspartate transaminase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Sus scrofa]] | [[Category: Sus scrofa]] | ||
| Line 27: | Line 28: | ||
[[Category: pig]] | [[Category: pig]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:51:07 2007'' |
Revision as of 09:46, 30 October 2007
|
REFINEMENT AND COMPARISON OF THE CRYSTAL STRUCTURES OF PIG CYTOSOLIC ASPARTATE AMINOTRANSFERASE AND ITS COMPLEX WITH 2-METHYLASPARTATE
Overview
Two high resolution crystal structures of cytosolic aspartate, aminotransferase from pig heart provide additional insights into the, stereochemical mechanism for ligand-induced conformational changes in this, enzyme. Structures of the homodimeric native structure and its complex, with the substrate analog 2-methylaspartate have been refined, respectively, with 1.74-A x-ray diffraction data to an R value of 0.170, and with 1.6-A data to an R value of 0.173. In the presence of, 2-methylaspartate, one of the subunits (subunit 1) shows a ligand-induced, conformational change that involves a large movement of the small domain, (residues 12-49 and 327-412) to produce a "closed" conformation. No such, transition is observed in the other subunit (subunit 2), because crystal, lattice contacts lock ... [(full description)]
About this Structure
1AJS is a [Protein complex] structure of sequences from [Sus scrofa] with PLA as [ligand]. Active as [Aspartate transaminase], with EC number [2.6.1.1]. Structure known Active Sites: ACT and AXT. Full crystallographic information is available from [OCA].
Reference
Refinement and comparisons of the crystal structures of pig cytosolic aspartate aminotransferase and its complex with 2-methylaspartate., Rhee S, Silva MM, Hyde CC, Rogers PH, Metzler CM, Metzler DE, Arnone A, J Biol Chem. 1997 Jul 11;272(28):17293-302. PMID:9211866
Page seeded by OCA on Tue Oct 30 11:51:07 2007
