6zqo

<table><tr><td colspan='2'>[[6zqo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Recombinant_vesicular_stomatitis_Indiana_virus_rVSV-G/GFP Recombinant vesicular stomatitis Indiana virus rVSV-G/GFP]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZQO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZQO FirstGlance]. <br>

== Function ==

[https://www.uniprot.org/uniprot/B7UCZ6_9RHAB B7UCZ6_9RHAB]

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== Publication Abstract from PubMed ==

For the whole GFP family, a few cases, when a single mutation in the chromophore environment strongly inhibits maturation, were described. Here we study EYFP-F165G - a variant of the enhanced yellow fluorescent protein - obtained by a single F165G replacement, and demonstrated multiple fluorescent states represented by the minor emission peaks in blue and yellow ranges (~470 and ~530 nm), and the major peak at ~330 nm. The latter has been assigned to tryptophan fluorescence, quenched due to excitation energy transfer to the mature chromophore in the parental EYFP protein. EYFP-F165G crystal structure revealed two general independent routes of post-translational chemistry, resulting in two main states of the polypeptide chain with the intact chromophore forming triad (~85%) and mature chromophore (~15%). Our experiments thus highlighted important stereochemical role of the 165th position strongly affecting spectral characteristics of the protein. On the basis of the determined EYFP-F165G three-dimensional structure, new variants with ~ 2-fold improved brightness were engineered.

Amino acid residue at the 165th position tunes EYFP chromophore maturation. A structure-based design.,Pletneva NV, Maksimov EG, Protasova EA, Mamontova AV, Simonyan TR, Ziganshin RH, Lukyanov KA, Muslinkina L, Pletnev S, Bogdanov AM, Pletnev VZ Comput Struct Biotechnol J. 2021 May 11;19:2950-2959. doi:, 10.1016/j.csbj.2021.05.017. eCollection 2021. PMID:34136094<ref>PMID:34136094</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 6zqo" style="background-color:#fffaf0;"></div>

== References ==

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[[Category: Recombinant vesicular stomatitis Indiana virus rVSV-G/GFP]]