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1pvu

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{{STRUCTURE_1pvu| PDB=1pvu | SCENE= }}
{{STRUCTURE_1pvu| PDB=1pvu | SCENE= }}
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'''THE CRYSTAL STRUCTURE OF PVUII ENDONUCLEASE REVEALS EXTENSIVE STRUCTURAL HOMOLOGIES TO ECORV'''
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===THE CRYSTAL STRUCTURE OF PVUII ENDONUCLEASE REVEALS EXTENSIVE STRUCTURAL HOMOLOGIES TO ECORV===
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==Overview==
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The crystal structure of the dimeric PvuII restriction endonuclease (R.PvuII) has been determined at a resolution of 2.4A. The protein has a mixed alpha/beta architecture and consists of two subdomains. Despite a lack of sequence homology, extensive structural similarities exist between one R.PvuII subdomain and the DNA-binding subdomain of EcoRV endonuclease (R.EcoRV); the dimerization subdomains are unrelated. Within the similar domains, flexible segments of R.PvuII are topologically equivalent to the DNA-binding turns of R.EcoRV; potential catalytic residues can be deduced from the structural similarities to R.EcoRV. Conformational flexibility is important for the interaction with DNA. A possible classification of endonuclease structures on the basis of the positions of the scissile phosphates is discussed.
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(as it appears on PubMed at http://www.pubmed.gov), where 7664066 is the PubMed ID number.
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{{ABSTRACT_PUBMED_7664066}}
==About this Structure==
==About this Structure==
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[[Category: Vlassi, M.]]
[[Category: Vlassi, M.]]
[[Category: Type ii restriction endonuclease]]
[[Category: Type ii restriction endonuclease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:32:43 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 04:36:21 2008''

Revision as of 01:36, 29 July 2008

Image:1pvu.png

Template:STRUCTURE 1pvu

THE CRYSTAL STRUCTURE OF PVUII ENDONUCLEASE REVEALS EXTENSIVE STRUCTURAL HOMOLOGIES TO ECORV

Template:ABSTRACT PUBMED 7664066

About this Structure

1PVU is a Single protein structure of sequence from Proteus vulgaris. Full crystallographic information is available from OCA.

Reference

Crystal structure of PvuII endonuclease reveals extensive structural homologies to EcoRV., Athanasiadis A, Vlassi M, Kotsifaki D, Tucker PA, Wilson KS, Kokkinidis M, Nat Struct Biol. 1994 Jul;1(7):469-75. PMID:7664066

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