7nyq
From Proteopedia
(Difference between revisions)
| Line 3: | Line 3: | ||
<StructureSection load='7nyq' size='340' side='right'caption='[[7nyq]], [[Resolution|resolution]] 1.60Å' scene=''> | <StructureSection load='7nyq' size='340' side='right'caption='[[7nyq]], [[Resolution|resolution]] 1.60Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'> | + | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NYQ FirstGlance]. <br> |
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nyq OCA], [https://pdbe.org/7nyq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nyq RCSB], [https://www.ebi.ac.uk/pdbsum/7nyq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nyq ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nyq OCA], [https://pdbe.org/7nyq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nyq RCSB], [https://www.ebi.ac.uk/pdbsum/7nyq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nyq ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| - | == Function == | ||
| - | [https://www.uniprot.org/uniprot/M9PH32_DROME M9PH32_DROME] | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | The TRIM-NHL protein Meiotic P26 (Mei-P26) acts as a regulator of cell fate in Drosophila Its activity is critical for ovarian germline stem cell maintenance, differentiation of oocytes, and spermatogenesis. Mei-P26 functions as a post-transcriptional regulator of gene expression; however, the molecular details of how its NHL domain selectively recognizes and regulates its mRNA targets have remained elusive. Here, we present the crystal structure of the Mei-P26 NHL domain at 1.6 A resolution and identify key amino acids that confer substrate specificity and distinguish Mei-P26 from closely related TRIM-NHL proteins. Furthermore, we identify mRNA targets of Mei-P26 in cultured Drosophila cells and show that Mei-P26 can act as either a repressor or activator of gene expression on different RNA targets. Our work reveals the molecular basis of RNA recognition by Mei-P26 and the fundamental functional differences between otherwise very similar TRIM-NHL proteins. | ||
| - | |||
| - | Molecular insights into RNA recognition and gene regulation by the TRIM-NHL protein Mei-P26.,Salerno-Kochan A, Horn A, Ghosh P, Nithin C, Koscielniak A, Meindl A, Strauss D, Krutyholowa R, Rossbach O, Bujnicki JM, Gaik M, Medenbach J, Glatt S Life Sci Alliance. 2022 May 5;5(8). pii: 5/8/e202201418. doi:, 10.26508/lsa.202201418. Print 2022 Aug. PMID:35512835<ref>PMID:35512835</ref> | ||
| - | |||
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 7nyq" style="background-color:#fffaf0;"></div> | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: Drosophila melanogaster]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Gaik M]] | [[Category: Gaik M]] | ||
Current revision
Crystal structure of the Mei-P26 NHL domain
| |||||||||||
