7o2y
From Proteopedia
(Difference between revisions)
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<StructureSection load='7o2y' size='340' side='right'caption='[[7o2y]], [[Resolution|resolution]] 2.50Å' scene=''> | <StructureSection load='7o2y' size='340' side='right'caption='[[7o2y]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'> | + | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O2Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O2Y FirstGlance]. <br> |
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BLA:BILIVERDINE+IX+ALPHA'>BLA</scene></td></tr> | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BLA:BILIVERDINE+IX+ALPHA'>BLA</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o2y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o2y OCA], [https://pdbe.org/7o2y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o2y RCSB], [https://www.ebi.ac.uk/pdbsum/7o2y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o2y ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o2y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o2y OCA], [https://pdbe.org/7o2y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o2y RCSB], [https://www.ebi.ac.uk/pdbsum/7o2y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o2y ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| - | == Function == | ||
| - | [https://www.uniprot.org/uniprot/A0A1D5B367_SANVI A0A1D5B367_SANVI] | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Biliverdin IX-alpha (BV), a tetrapyrrole, is found ubiquitously in most living organisms. It functions as a metabolite, pigment, and signaling compound. While BV is known to bind to diverse protein families such as heme-metabolizing enzymes and phytochromes, not many BV-bound lipocalins (ubiquitous, small lipid-binding proteins) have been studied. The molecular basis of binding and conformational selectivity of BV in lipocalins remains unexplained. Sandercyanin (SFP)-BV complex is a blue lipocalin protein present in the mucus of the Canadian walleye (Stizostedion vitreum). In this study, we present the structures and binding modes of BV to SFP. Using a combination of designed site-directed mutations, X-ray crystallography, UV/VIS, and resonance Raman spectroscopy, we have identified multiple conformations of BV that are stabilized in the binding pocket of SFP. In complex with the protein, these conformers generate varied spectroscopic signatures both in their absorption and fluorescence spectra. We show that despite no covalent anchor, structural heterogeneity of the chromophore is primarily driven by the D-ring pyrrole of BV. Our work shows how conformational promiscuity of BV is correlated to the rearrangement of amino acids in the protein matrix leading to modulation of spectral properties. | ||
| - | |||
| - | Modulation of biliverdin dynamics and spectral properties by Sandercyanin.,Ghosh S, Mondal S, Yadav K, Aggarwal S, Schaefer WF, Narayana C, Subramanian R RSC Adv. 2022 Jul 13;12(31):20296-20304. doi: 10.1039/d2ra02880h. eCollection, 2022 Jul 6. PMID:35919616<ref>PMID:35919616</ref> | ||
| - | |||
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 7o2y" style="background-color:#fffaf0;"></div> | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Sander vitreus]] | ||
[[Category: Ghosh S]] | [[Category: Ghosh S]] | ||
[[Category: Ramaswamy S]] | [[Category: Ramaswamy S]] | ||
[[Category: Yadav K]] | [[Category: Yadav K]] | ||
Current revision
Sandercyanin Fluorescent Protein variant V71E bound to biliverdin IX-alpha
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