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1px4

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[[Image:1px4.jpg|left|200px]]
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{{STRUCTURE_1px4| PDB=1px4 | SCENE= }}
{{STRUCTURE_1px4| PDB=1px4 | SCENE= }}
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'''E. COLI (LACZ) BETA-GALACTOSIDASE (G794A) WITH IPTG BOUND'''
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===E. COLI (LACZ) BETA-GALACTOSIDASE (G794A) WITH IPTG BOUND===
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==Overview==
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The open-closed conformational switch in the active site of Escherichia coli beta-galactosidase was studied by X-ray crystallography and enzyme kinetics. Replacement of Gly794 by alanine causes the apoenzyme to adopt the closed rather than the open conformation. Binding of the competitive inhibitor isopropyl thio-beta-D-galactoside (IPTG) requires the mutant enzyme to adopt its less favored open conformation, weakening affinity relative to wild type. In contrast, transition-state inhibitors bind to the enzyme in the closed conformation, which is favored for the mutant, and display increased affinity relative to wild type. Changes in affinity suggest that the free energy difference between the closed and open forms is 1-2 kcal/mol. By favoring the closed conformation, the substitution moves the resting state of the enzyme along the reaction coordinate relative to the native enzyme and destabilizes the ground state relative to the first transition state. The result is that the rate constant for galactosylation is increased but degalactosylation is slower. The covalent intermediate may be better stabilized than the second transition state. The substitution also results in better binding of glucose to both the free and the galactosylated enzyme. However, transgalactosylation with glucose to produce allolactose (the inducer of the lac operon) is slower with the mutant than with the native enzyme. This suggests either that the glucose is misaligned for the reaction or that the galactosylated enzyme with glucose bound is stabilized relative to the transition state for transgalactosylation.
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{{ABSTRACT_PUBMED_14621996}}
==About this Structure==
==About this Structure==
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[[Category: Matthews, B W.]]
[[Category: Matthews, B W.]]
[[Category: Loop conformation]]
[[Category: Loop conformation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:35:25 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 18:40:10 2008''

Revision as of 15:40, 28 July 2008

Image:1px4.png

Template:STRUCTURE 1px4

E. COLI (LACZ) BETA-GALACTOSIDASE (G794A) WITH IPTG BOUND

Template:ABSTRACT PUBMED 14621996

About this Structure

1PX4 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structural basis for the altered activity of Gly794 variants of Escherichia coli beta-galactosidase., Juers DH, Hakda S, Matthews BW, Huber RE, Biochemistry. 2003 Nov 25;42(46):13505-11. PMID:14621996

Page seeded by OCA on Mon Jul 28 18:40:10 2008

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