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| - | [[Image:1pxd.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1pxd| PDB=1pxd | SCENE= }} | | {{STRUCTURE_1pxd| PDB=1pxd | SCENE= }} |
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| - | '''Crystal structure of the complex of jacalin with meso-tetrasulphonatophenylporphyrin.'''
| + | ===Crystal structure of the complex of jacalin with meso-tetrasulphonatophenylporphyrin.=== |
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| - | ==Overview==
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| - | The crystal structure of the complex of meso-tetrasulfonatophenylporphyrin (H(2)TPPS) with jack fruit (Artocarpus integriflora) agglutinin (jacalin) has been determined at 1.8 A resolution. A porphyrin pair is sandwiched between two symmetry-related jacalin monomers in the crystal, leading to a cross-linking network of protein molecules. Apart from the stacking interactions, H(2)TPPS also forms hydrogen bonds, some involving water bridges, with jacalin at the carbohydrate-binding site. The residues that are involved in rendering galactopyranoside specificity to jacalin undergo conformational adjustments in order to accommodate the H(2)TPPS molecule. The water molecules at the carbohydrate-binding site of jacalin cement the jacalin-porphyrin interactions, optimizing their complementarity. Interactions of porphyrin with jacalin are relatively weak compared with those observed between galactopyranoside and jacalin, perhaps because the former largely involves water-mediated hydrogen bonds. While H(2)TPPS binds to jacalin at the carbohydrate-binding site as in the case of ConA, its mode of interaction with jacalin is very different. H(2)TPPS does not enter the carbohydrate-binding cavity of jacalin. Instead, it sits over the binding site. While the porphyrin binding is mediated by replicating the hydrogen-bonding network of mannopyranoside through the sulfonate atoms in the case of ConA, the plasticity associated with the carbohydrate-binding site accommodates the pluripotent porphyrin molecule in the case of jacalin through an entirely different set of interactions. | + | The line below this paragraph, {{ABSTRACT_PUBMED_14747704}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 14747704 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_14747704}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Lectin]] | | [[Category: Lectin]] |
| | [[Category: Porphyrin]] | | [[Category: Porphyrin]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:35:54 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:06:34 2008'' |
Revision as of 00:06, 29 July 2008
Template:STRUCTURE 1pxd
Crystal structure of the complex of jacalin with meso-tetrasulphonatophenylporphyrin.
Template:ABSTRACT PUBMED 14747704
About this Structure
1PXD is a Protein complex structure of sequences from Artocarpus integer. Full crystallographic information is available from OCA.
Reference
Porphyrin binding to jacalin is facilitated by the inherent plasticity of the carbohydrate-binding site: novel mode of lectin-ligand interaction., Goel M, Anuradha P, Kaur KJ, Maiya BG, Swamy MJ, Salunke DM, Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):281-8. Epub 2004, Jan 23. PMID:14747704
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