1pxv

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[[Image:1pxv.jpg|left|200px]]
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[[Image:1pxv.png|left|200px]]
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{{STRUCTURE_1pxv| PDB=1pxv | SCENE= }}
{{STRUCTURE_1pxv| PDB=1pxv | SCENE= }}
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'''The staphostatin-staphopain complex: a forward binding inhibitor in complex with its target cysteine protease'''
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===The staphostatin-staphopain complex: a forward binding inhibitor in complex with its target cysteine protease===
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==Overview==
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Staphostatins are the endogenous inhibitors of the major secreted cysteine proteases of Staphylococcus aureus, the staphopains. Our recent crystal structure of staphostatin B has shown that this inhibitor forms a mixed, eight-stranded beta-barrel with statistically significant similarity to lipocalins, but not to cystatins. We now present the 1.8-A crystal structure of staphostatin B in complex with an inactive mutant of its target protease. The complex is held together through extensive interactions and buries a total surface area of 2300 A2. Unexpectedly for a cysteine protease inhibitor, staphostatin B binds to staphopain B in an almost substrate-like manner. The inhibitor polypeptide chain runs through the protease active site cleft in the forward direction, with residues IG-TS in P2 to P2' positions. Both in the free and complexed forms, the P1 glycine residue of the inhibitor is in a main chain conformation only accessible to glycines. Mutations in this residue lead to a loss of affinity of the inhibitor for protease and convert the inhibitor into a substrate.
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The line below this paragraph, {{ABSTRACT_PUBMED_12874290}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 12874290 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12874290}}
==About this Structure==
==About this Structure==
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[[Category: Rzychon, M.]]
[[Category: Rzychon, M.]]
[[Category: Cysteine protease inhibitor]]
[[Category: Cysteine protease inhibitor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:37:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:51:36 2008''

Revision as of 12:51, 28 July 2008

Image:1pxv.png

Template:STRUCTURE 1pxv

The staphostatin-staphopain complex: a forward binding inhibitor in complex with its target cysteine protease

Template:ABSTRACT PUBMED 12874290

About this Structure

1PXV is a Protein complex structure of sequences from Staphylococcus aureus. Full crystallographic information is available from OCA.

Reference

The Staphostatin-staphopain complex: a forward binding inhibitor in complex with its target cysteine protease., Filipek R, Rzychon M, Oleksy A, Gruca M, Dubin A, Potempa J, Bochtler M, J Biol Chem. 2003 Oct 17;278(42):40959-66. Epub 2003 Jul 21. PMID:12874290

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