1rzd
From Proteopedia
(New page: 200px<br /> <applet load="1rzd" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rzd, resolution 1.9Å" /> '''X-RAY ANALYSIS OF ME...) |
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caption="1rzd, resolution 1.9Å" /> | caption="1rzd, resolution 1.9Å" /> | ||
'''X-RAY ANALYSIS OF METAL SUBSTITUTED HUMAN CARBONIC ANHYDRASE II DERIVATIVES'''<br /> | '''X-RAY ANALYSIS OF METAL SUBSTITUTED HUMAN CARBONIC ANHYDRASE II DERIVATIVES'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1RZD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MN and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] Full crystallographic information is available from [http:// | + | 1RZD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZD OCA]. |
==Reference== | ==Reference== | ||
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[[Category: lyase(oxo-acid)]] | [[Category: lyase(oxo-acid)]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:51:08 2008'' |
Revision as of 14:51, 15 February 2008
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X-RAY ANALYSIS OF METAL SUBSTITUTED HUMAN CARBONIC ANHYDRASE II DERIVATIVES
Contents |
Overview
Metal-substituted crystals of human carbonic anhydrase II belonging to, space group P2(1) with cell dimensions a = 42.7, b = 41.7, c = 73.0 A and, beta = 104.6 degrees were analyzed crystallographically. The resolution, limit ranged from 1.82 to 1.92 A with high completeness (86.2-90.7%)., Cobalt(II)-substituted carbonic anhydrase has a tetrahedral coordination, around the metal both at pH 6 and pH 7.8, similar to the native zinc, enzyme. In contrast, the catalytically inactive copper(II), nickel(II) and, manganese(II) derivatives showed increased coordination number around the, metal ion. Whereas the copper is best described as penta-coordinated, the, nickel and manganese are best described as hexa-coordinated. The results, are briefly compared with spectroscopic observations and our current view, on carbonic anhydrase catalysis.
Disease
Known disease associated with this structure: Osteopetrosis, autosomal recessive 3, with renal tubular acidosis OMIM:[611492]
About this Structure
1RZD is a Single protein structure of sequence from Homo sapiens with and as ligands. Active as Carbonate dehydratase, with EC number 4.2.1.1 Full crystallographic information is available from OCA.
Reference
X-ray analysis of metal-substituted human carbonic anhydrase II derivatives., Hakansson K, Wehnert A, Liljas A, Acta Crystallogr D Biol Crystallogr. 1994 Jan 1;50(Pt 1):93-100. PMID:15299481
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